Analysis And Study Of Non-uv Absorption Drugs Based On Charge Transfer Reaction

Charge transfer complex(CTC) is a class of complex between Electron Donor and Acceptor through charge transfer reaction. The spectral analysis using charge transfer complex formation is called charge transfer spectral analysis which has a wide range of applications in chemistry, biochemistry and medicine, especially in the quantitative analysis of drugs. Its application in the quantitative analysis of drugs is mainly utilize the CTC which is often accompanied with a specific color and maximum absorption to establish spectrophotometric determination of drugs. The application of Charge transfer spectral analysis in pharmaceutical analysis is the research focus in recent years, focusing on applications in the quinolones, sulfonamides, amino acids, cephalosporins and other drugs analysis.In this thesis, spectrophotometric method was applied to determine the Non UV absorption drugs. In addition, new methods of HPLC determination of Non UV absorption drugs based on charge transfer reaction were established through the application of the Charge Transfer Theory into pre column derivatization HPLC method.The first part summarized the concept of CTC, the formation of CTC, the classification of electron donor and acceptor, as well as the application of CTC in biomedical and chemical fields. In the second to the fourth parts, the factors affecting the charge transfer reaction, such as the type of electron acceptor reagent, the charge transfer reaction medium, the pH and amount of buffer solution, charge transfer reaction temperature and time, the amount of electron acceptor reagent and so on were studied. After that, spectrophotometric method was established to determine the content of agmatine sulfate salt. Spectrophotometric method and HPLC method based on charge transfer reaction were established to determine the content of sisomicin sulfate. HPLC method based on charge transfer reaction was established to determine the content of carbocisteine. Besides, the composition, molar absorptivity, association constant of complexes and CTC formation mechanism were studied. The fifth part was the conclusion and prospection. These two methods were simple, accurate, reproducible, satisfactory and could be used to determine non UV absorption drugs.