Constructing The Canine Model Of Brugada Syndrome And Electrophysiological Effects Of Cilostazol

Objective: In this research, we examine the electrophysiological characteristics of the dogs in a canine model of Brugada syndrome and the electrophysiological effects produced by Cilostazol, a Phosphodiesterase III inhibitor, on these dogs.Method: A total of 20 dogs, after arterially perfused right ventricular wedge preparations made, were firstly observed for the electrophysiological characters of three certain solutions used in the model. After being washed out and perfused with Pinacidil solution of 2ummol/L, Terfenadine solution of 5ummol/L and Pilsicainide solution of 5ummol/L at the same time, 16 dogs of the 20 were found eligible for the canine model of Brugada syndrome, and the electrophysiological parameters of their wedge preparations and ventricular arrhythmia events, spontaneous or induced, were recorded. These 16 dogs were then randomly divided into two groups, i.e., model group and intervention group, and we continued to inject the perfusion of the aforesaid three solutions to the 8 dogs in the model group while the 8 dogs in the intervention group were perfused with Cilostazol to explore its effects on the electrophysiological parameters and the incidence of ventricular arrhythmias. Result: 1. The perfusion of Pinacidil,Terfenadine and Pilsicainide solutions proved to be reliable in constructing the model of Brugada syndrome. 2. The transmural dispersion of repolarization (TDR) and epicardial dispersion of repolarization both increased in the Brugada model, suggesting that increased dispersion of repolarization might be the main stroma of ventricular arrhythmias. 3 in the intervention group TDR and epicardial DR was reduced and the incidence of ventricular arrhythmias decreased significantly in the intervention group as compared with that in the model group (p<0.05).Conclusion: Cilostazol, through decreasing the widened transmural dispersion of repolarization of arterially perfused canine preparations and partially offsetting the latter's effects, can effectively prevent and reduce the incidence of ventricular arrhythmia in the canine model of Brugada syndrome.