The Effect Of Nerve Growth Factor Injected In Cerebral Ventricle On The Behavior Change Of Hydrocephalus Young Rats

Object:To study the effect of nerve growth factor injected in cerebral ventricle on the behavior change of hydrocephalus young rats.Methods:301-month-old male Wistar rats, all treated by injecting white clay into cisterna magna to prepare the models of communicating hydrocephalus, were verified 1 month later by MRI. After the models prepared, the rats were randomly divided into three groups,10 rats each group. The experimental group were injected NGF 0.3ml (1000U, for 3 days consecutively) in cerebral ventricle; the placebo group were injected 0.3ml physiological saline (for 3 days consecutively) in cerebral ventricle, the control group were not to have any treatment.2 weeks later, we studied the three groups of rats'behavior respectively by Morris water maze and open-field analysis, then the rats were killed after experiment. And we did HE staining and immunohistochemistry to observe the structural changes in hippocampus of rats and expression of S-100(3 and GFAP.Results:the rats in placebo group and control group had the longer swimming time and more errors in water maze and the less capability in spatial cognition and adaptability to new environment than rats in the experimental group. Hippocampus of rats in each group had positive expression of GFAP, and there were significant differences between experimental group and the placebo group, also the control group. We can see S-100β-positive cells in hippocampus of rats in each group. A large number of positive stained cells can be seen in hippocampus of the placebo group and control group rats, with significantly different to the experimental group.Conclusion:The placebo group and control group of young rats had less learning, memorizing and spatial cognition ability and activity than the experimental group, showing that injection of nerve growth factor in cerebral ventricle has protective function to the hydrocephalus cerebellum damage.