Study On The Correlation Of Methylation Of Cadm1, Tfpi2 With Cervical Cancer In Xinjiang Uygur And Han

Objectives:To investigate the correlations of Promoter methylation of CADM1, TFPI2 with Cervical cancer and HPV16 infection in Xinjiang Uygur and Han women.Methods:Promoter methylation status of CADM1 and TFPI2 were Detected by MALDI-TOF MS spectrometer in tissues of Xinjiang Uygur women Cervical cancer(n=55), CIN2/3 lesions (n=36), CIN1 lesions (n=11), cervical healthy control (n=21) and Han women Cervical cancer (n=58), CIN2/3 lesions (n=57), CIN1 lesions (n=36), cervical healthy control (n=28).HPV16 DNA was detected by PCR in the cases of Xinjiang Uygur and Han women.Results:(1) Methylation Status of CADM1:the mean methylation levels of CpG₂.3.4.5,CpG₇,CpG₁1,CpG₁3.14,CpG₁5 were significantly increased in cervical carcinomas group compared with CINs and healthy control in Uygur women(p<0.05). The methylation state of CpG₁,CpG₈,CpG₁1,CpG₁5 in cervical carcinoma were significant higher than CINs and healthy control in Han women(p<0.05).No significant diference in methylation between CINs and healthy control in Uygur and Han women.(p>0.05). The methylation state of CADM1 was not correlate with lymph node metastases(p >0.05). (2) Methylation Status of TFPI2:the mean methylation levels of CpG₁5,CpG₁8.19,CpG₃1 were significantly increased in cervical carcinomas group compared with CINs and healthy controlin Uygur women(p<0.05). The methylation state of CpG₂1.22,CpG₃1 in cervical carcinoma were significant higher than CINs and healthy control in Han women (p<0.05).No significant diference between CINs and healthy control in Xinjiang Uygur and Han women.(p>0.05). The methylation state of TFPI2 was not correlate with lymph node metastases(p>0.05).(3) There were several commom CpG sites and some different CpG sites with CADM1,TFPI2 promoter hypermethylation in ICC of Xinjiang Uygur and Han women.(4) HPV16 infection and methylation:The methylation states of CADM1_CpG₁,CpG₈,CpG₁1,CpG₁3.14,CpG₁5 and TFPI2_CpG₁5,CpG₂3 were significantly higher in HPV 16 positive cervical carcinomas than in HPV negative samples in Uygur women. The methylation states of CADM1_CpG₁1,GpG₁5 were significantly higher in HPV 16 positive cervical carcinomas than in HPV negative samples in Han women.Conclusions:(1) Hypermethylation of CpG₂.3.4.5,CpG₇,CpG₁1,CpG₁3.14,CpG₁5 may contribute to the promotion to the development of cervical cancer in Uygur women,Hypermethylation of CpG₁,CpG₈,CpG₁1,CpG₁5 may contribute to the promotion to the development of cervical cancer in Han women. (2) Hypermethylation of TFPI1_CpG₁5,CpG₁8.19,CpG₃1 may contribute to the promotion to the development of cervical cancer in Uygur women,Hypermethylation of CpG₂1.22,CpG₃1 may contribute to the promotion to the development of cervical cancer in Han women. (3) The differenceof CpG site methylation of CADM 1 or TFPI2 may affect the risk of cervical cancer in Xinjiang Uygur and Han women. (4) The hypermethylation states of CADM1 CpG₈, CpG₁1, CpG 15, TFPI2_CpG₁5,CpG₂3 in Uygur women, and CADM1_CpG₁1,CpG₁5 in Han women may be required for a HR-HPV-infected cell to progress to invasive carcinoma,...