| Background:To explore effects of periosteum on healing of bone-tendon junction and provide experimental evidences on treating injuries of bone-tendon junction via periosteum accelerating cellular proliferation and healing.Methods:Standard Animal model of partial patellectomy in 36 mature female New Zealand white rabbits (18-weeks-old) were established and randomly classified into 2 groups. The experimental group was planted into by periosteum while the control group was only operated. The Quadriceps Muscle-Patella-Patellar Tendon (QMPPT) were harvested at weeks 4,8,12 postoperatively for histologic (standard H&E stain,Safranin'0 stain and Masson strain). MRI test before the animals were killed. Periosteum group and control group were used absorptance value to comparison. The result analysed with SPSS13.0 statistical software.Findings:Gross observation found the experimental group healing faster than control. Histologically showed the experimental group at weeks 4,8,12 more improved tissue integration, characterized by trabecular bone expansion from the remaining patellar and regeneration of fibrocartilage zone at the patellar tendon-patellar healing junction. The result means that the tissue healing of the experimental group was quicker than that of the control group. Imaging studies also confirm this view. Safranin'O staining staining of absorptance value detect found pstoperative 4 weeks the periosteum group value is 586.34±32.91, then the control group is 198.23±16.22, the two groups had significant difference (P<0.05). Followed by pstoperative 4 weeks the periosteum group value is 2697.67±87.86, the control group is 743.73±71.61. The two groups had significant difference (P<0.05)。In the last, the result of two groups in pstoperative 12 weeks had no significant difference (P>0.05).Interpreation:periosteum could accelerate celluar proliferation, matrix synthesis, new bone and fibrocartilage transitional zone formation and accelerate bone-tendon junction healing. |
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