| UV radiation comes from the sun in the 200-400nm band and it has the dual effect to human skin. On the one hand the appropriate ultraviolet radiation can accelerate the synthesis of vitamin D of body, On the other hand, UVB band (280-320nm) radiation to the skin, prone to sunburn and pigmentation and other acute injury; UVA band (320-400nm) with high energy and penetrating, long-term cumulative will damage the dermal layer of skin, resulting in the formation of skin photoaging; UVB and UVA interaction was likely to cause skin photosensitive reactions. According to the mechanisms of photosensitivity , 20 plant extracts with anti-photosensitive effect were screened. The main functional ingredient content, extraction and process optimization, function pathway and mechanism were also studied.In this paper, a relative less time and money consuming convenient method was developed to detect the damage of UV-A by red blood cell hemolysis, the hemoglobin of the injured red blood cell, and then calculate hemolytic rate. The experiments seeing about the effects on the model under the conditions of ultraviolet light or photo sensitizer existence alone and the different exposure time and the different drug concentration. The results show that red blood cell hemolysis cannot happen only under ultraviolet light radiation without Chlorpromazine nor only Chlorpromazine without ultraviolet light radiation. Under the condition of the chlorpromazine content is 1mg/3mL in red blood cell, 50% hemolysis can be resulted after they were radiated by ultraviolet for about 30min, and 100% hemolysis after 60min. This model can be applied to photosensitive material testing and screening of anti-photosensitive material.20 kinds of Chinese medicines and plant extracts were screened initially by UV scanning profiles, ginkgo biloba, grape seed, tea polyphenols, schisandra extract and so on had excellent UV absorption. The stimulation of several extract were detected by hemolysis experiments, hemolytic rate below 10%, proving the several extract have no stimulation. Ginkgo biloba and cactus extract which good anti-allergic were screened by stability test, ginkgo biloba extract in which the strongest UV absorption. Chemical composition analysis revealed that flavonoids was the major components.According to flavonoid solubility in water and industrial production needs, deionized water was selected as solvent extraction of the active ingredient. In the case of single factor, water to solid ratio 10~20, temperature 80~100℃, time 2~4h, the higher flavonoids rate was obtained. Based on the single factor experiments results and practical operation, the extraction procedure was optimized using Box-Behnken design and Surface Response Methodology (RSM).The obtained optimal parameters of the extraction procedure of flavonoids were: water to solid ratio 15:1, time 4 h, temperature 90℃, and the average extract content of flavonoids 6.22 mg/mL.Ion exchange resin was used to desalinize mixture extract. Ionic columns are 001×7-D309 ion exchange resin, the sample volume 5:1, sample flow rate was 4BV ? h -1 , the results was reduce the conductivity of liquid more than 8000μs/cm to 100μs/cm below a n d desalting effect was remarkable. The compound final electrical conductivity lower than 800μs/cm, more than 30% soluble solids, color, pH value both to meet the requirement, can be used as a cosmetic additive.The experimental anti-allergy and anti-irritation compound solution had good anti-allergic and free radicals clearance effects. Under 4.0% dosage, the hyaluronic acid enzyme inhibition rate, O 2- , ? OH and DPPH radicals clearance rates were 63.2%, 80.7%, 80.5% and 56.7%, respectively. The light hemolysis experiments show that adding 10.0% compound solution can protect red blood cells do not hemolysis within UV irradiation 60 min. 30min UV irradiation within the mixture add 5.0% solution can protect red blood cells hemolysis do not occur. Results showed that the anti-photosensitive liquid compound has good inhibition effect of UV radiation damage the cell membrane.By the model of DNA damaged detect the inhibitory effect of UV-damage DNA of anti-photosensitive compound solution. The results show that good inhibition on the DNA damage caused by UVA when the volume of compound solution in the concentration of added 0.1g/mL. When the concentration of 0.2g/mL, the inhibitory effect of UVA induced DNA damage was more obvious. Gel electrophoresis showed that the DNA was degradated and damaged w i t h i n 10min UVA radiation and the bands ghosting; When UVA irradiation 20min, DNA degradation was gradual expansion and more severe tailing; And the DNA bands added anti-photosensitive compound liquid significantly more clear and no tail bands than the damaged DNA. Experimental results showed anti-photosensitive compound liquid can inhibit the UVA caused DNA damage. |
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