Am Fungi And Flavonoids With Amorpha Interaction And Ddrt-pcr Screening Of Specific Fragment System

There are extensive infectivity between arbuscular mycorrhiza and plants, which including 80% of flowering plants, fern and bryophyte in the world. It had been demonstrated that arbuscular mycorrhiza can promote the absorption and exploitation of plant to mineral element, improve the nutritional status of plants, strengthen the antireversion force of plants, increase the yield and quality of plants, accelerate the survival rate of transplants. At present, the identification signals on the symbiosis of the international research study on the plant - the hot microbial symbiosis on the symbiotic identification signals of the initial study focused on Rhizobium and the interaction between the host plant identification, and the mycorrhizal symbiosis of mutual recognition Signal is only infer that the host and leguminous plants are herbaceous plants were used.In this paper, , Amorpha fruticosa was plant with flavonoid and AMF, the infection rate and variance of yield was observed regularly. The results showed that the AMF could conspicuous promote the growth of Amorpha fruticosa,Infection rate of about 90% AM fungi inoculation of the high﹑ Amorpha fruticosa stem and root fresh weight than unvaccinated Amorpha fruticosa higher than the average 2 cm﹑ 2 cm and 0.1 g.The flavonoid can also promote the growth of Amorpha fruticosa .Its high﹑roots stem fresh weight and have increased by 4 cm﹑ 4 cm and 0.1 g.And become more and more conspicuous along with the increase of flavonoid so that the flavonoid was take on some ability of signals in the process of infection between AMF to Amorpha fruticosa.In this paper, the technology of mRNA differential display was used for separate the specificity gene-fragment between non-innoculation and mon-innoculation AM fungi. The gene that was refer to the signal was detected by the technology of molecular biology and several specificity gene-fragment separated.