Campanulaceae Germplasm Resources Studies

The experiment was integrated Jilin Provincial Department for Science and TechnologyBasic research Department about the project of application of molecular mark technology inPlatycodon grandiflorus collection. My discussion group collected 56 germplasm resourcesof P. grandiflourus from China,Japan and Southern Korea(table 5-1) and established thegarden of germplasm resources of P. grandiflourus. With the cultural and wild species,observed variant types of the main agronomic characters and fingerprinted research for 56gcrmplasm resources of P. grandiflourus by RAPD. Arm for analysing genetic diversity,providing genetic marker for identifying germplasm resources and providing original materialfor breeding of new variety of P. grandiflourus.Measure the germination rate of P. grandiflourus seeds in market. The results showedthat the seeds from markets had low germinative ability. The main reason was that thematuration degree of seed was of a sort, relaxed in choiceness, poor saved condition and themuch longer preservation time after harvest.To observe the seed appearance and seed color of the germplasm resources ofP.grandiflourus form different origin. The results showed that the seed appearance, color andseed wing color etc had the diversity. These characters were distinct difference among thegermplasm resources from different origin or the same germplasm resource and differentplants. But, these characters on these same plant were complete same. Hereby, author thoughtthat these characters were concerned with genetic factor and qua genetic markers identifieddifferent germplasm resources of P. grandiflourus.Observed the plant height, stem thick, leaf number of main stem, the fresh ofaboveground, the length of main root, the side root number, root thick and the single rootweight of 50 germplasm resources of Pgrandiflourus. The results showed that it was obviousvariation range on plant height etc about 8 main agronomic characters. It was considerableutilize foreground in breeding of new variety for P.grandiflourus. The results of correlation,multiple regression analysis and path analysis showed that root thick and stem thick was theimportant factors for the single root weight. It become the main guide line of individual plantselection after breeding of new variety for Pgrandiflourus.The total DNA from young leaves of the P.grandiflourus of Tonghua was successfullyextracted by using the developed CTAB method. The results showed that the most optimalreacton system of RAPD is. 2Ong template DNA,dNTP concentration 150μL, 0.3μmol/Lprimer, MgCl₂ 2.0mmol, 1U Taq polymerase, 10×Taq polymerase buffer at 2.0μL in the 20μL reacton volume.The objective of this study was to analyze the genetic diversity with 25random primers among the 56 samples of P. grandiflourus from different countries or regionsby RAPD analysis. The results showed that the 56 samples of P. grandiflourus were mainlyclustered according to geographical distribution and were divided into eight groups at geneticdistance 0.275.