In Vitro Cytotoxicity Testing Of Glyphosate And Monosultap Using Different Ploidy Loach Fin Cell Lines

60-70 generation of diploid loach fin cell line(DIMF) and triploid loach fin cell line(TRMF) were used to study different concentrations of glyphosate and monosultap toxicity in vitro. The 24h-IC50 of DIMF and TRMF were 333.79mg/L and 384.91mg/L by glyphosate and 119.73mg/L and 146.26mg/L by monosultap respectively, which were measured with MTT. The glyphosate and monosultap sensitivity of DIMF was higher than TRMF obviously. After exposure of glyphosate and monosultap, the cellular morphology showed the phenomenon with elongation, vacuolization and exfoliation. The results of two kinds of cell lines enzyme activity determination showed while the glyphosate concentration was 0~300mg/L, the activity of SOD and GST were increased with the increasing concentration then gradually decreased. The activity of ACh E was negatively correlated with the concentration of glyphosate.monosultap concentration was 0~100mg/L, the activity of SOD and GST were increased with the increasing concentration then gradually decreased. The activity of ACh E was negatively correlated with the concentration of monosultap. And the three enzymes of TRMF were higher than DIMF. Micronucleus test showed that 100mg/L glyphosate and 50mg/L monosultap can make the nucleus produces damage and then form the micronucleus, the rate of micronucleus increased with the increase of glyphosate and monosultap concentration, when glyphosate and monosultap concentration were 500mg/L and 200mg/L that were maximum, the micronucleus rate of DIMF and TRMF were 4.3‰ by glyphosate and which were 3.3‰ and 3.7‰ by monosultap. The ultrastructural observation show that no significant difference between DIMF and TRMF organelles in control group. After exposure of glyphosate and monosultap, DIMF and TRMF presented similar pathological changes, the both showed chromatin condensation, margination and cell nucleus decomposition. The number of organelles have reduced, including mitochondria, ribosomes and endoplasmic reticulum. Vacuoles and apoptotic bodies occured in cells which indicated cell poptosis has occurred. This paper studied not only the difference betweent different ploidy loach fin cell lines and mechanisms of toxicity by glyphosate and monosultap, but also laid a foundation for establishing pesticide monitoring system in vitro.