Design Of Tilapia Collagen Peptide Pilot Plant And Its Production

Collagen peptide not only has small molecular which can be easily digested and absorbed, but also has unique biological activities, especially in areas such as antihypentensive, antioxidant, anti-cancer. Therefore, they have good prospects in medicine, health and beauty areas. This study which was based on the business needs designed a GMP pilot plant for collagen peptide complied with the production requirments of functional products, and tiapia collagen as raw materials, combined with bio-enzymatic technology, membrane technology, modern biotechnology to a preparation of biologically ativity collagen peptide in the GMP pilot plant.Firstly, a GMP pilot plant of collagen peptide was designed. Technology testify has accomplished according to characteristics of collagen peptides, and on the basis of it the material balance, device selection and the usage amount of the water and electricity has finished. In this design, The wax and wane balance point was 49.3 tons, the conducting safety point was 52.1%, and the recycle period of investment was expected about 4.4 year. After the accomplishment of the design specification, large number of drawing work was done in this design as follows: plane figure of main workshop, flow diagram of people and materials, equipment flowsheet and equipment distribution of the pilot plant.Secondly, optimum enzyme condition by shrimp enzyme was at 37 ℃, p H 8.0, with enzyme dosage of 0.5% and incubation for 3 h, with tialapia collagen as object. The hydrolysis condition by alcalase was at 45 ℃, p H 9.0, with enzyme dosage of 0.1% and incubation for 3 h. Meanwhile, the molecular weight distribution of these two kinds hydrolysates had been measured, which mostly distributed under 2 k Da. Based on the optimized hydrolysis condition, the pilot production had conducted. The pilot production process flow of the tilapia collagen peptide was enzymatic hydrolysis, microfiltraction, ultrafiltration, nanofiltration, spray drying and package. Strictly control was needed in the parameters and sanitary condition of the operation steps, rigidly following the standards of GMP. In the membrane separation process, membrane flux decreases with time prolonging.Lastly, analysis of basic property of the product has been finished. The tilapia collagen peptide prepared by shrimp head enzyme has significant high activity in ACE inhibitory, with IC50 of 0.52 mg/m L. And the peptide prepared by alcalase has high antioxidant activity, with IC50 of 5.7 mg/m L by analysis of DPPH radical scavenging capacity. The molecular weight of collagen peptide prepared by these two kinds enzymes was similar, with mostly distributing under 1 k Da. And the product of tilapia collagen peptides sensory evaluation is white, tasteless, no visible impurity by the sense of sight. As to the functional characteristics, products had low oil-blotting, high hygroscopicity, water holding capacity and high solubility. The molecular weight of collagen peptide products were mostly distributed under 1 k Da. The activity of ACE inhibitory of the products was decreased by the simulated gastrointestinal digestion, with the IC50 of 0.85 mg/m L. However, its antihypertensive effect was still high.A GMP pilot plant of collagen peptide was designed. In the pilot plant, the pilot production of tilapia collagen was completed, and then the analysis of basic property of it had been finished. This pilot plant design of collagen peptide had filled in the blanks. This study had laid solid foundation for the industrialization of tilapia collagen peptide, and had provided a useful reference for high efficient utilization of tilapia by-products, as well as the scientific basis for the development of functional foods.