Preparation And Activity Of Fucoxanthin Produced By Sargassum Herneri(tarn) Ag

This paper took Sargassum horneri as the material for the study of fucoxanthin extraction and its physiological activity. Optimize the extraction conditions and the antioxidant and anti-inflammatory ability of fucoxanthin was evaluated. There are the main findings: 1. Extraction and purification of fucoxanthinCompare the effects of conventional organic solvent and ultrasonic technique. The best extraction technique was acquired by using single factor method and the orthogonal approach. The results show that:(1) 70% ethanol was the most effective one for the extraction;(2) the best way of conventional organic solvent extraction were as follows: the temperature was 70℃, the ratio of S/M(solvent volume/material weight) was 68.3ml/g and the seaweed was extracted in 127 min. The highest extraction rate was 0.72mg/g(calculated with dry Sargassum herneri);(3) The highest extraction rates with ultrasonic was 0.70mg/g when the temperature was 70℃, the ratio of S/M was 50ml/g and the time was 50 min. 2. Effect of fucoxanthin on antioxidative activity in vitroFirst, the topic studied the ability of fucoxanthin to scavenge free radical and its reducing power. The results indicated that free radical clearance rate were very high by fucoxanthin in a dose-dependent manner. When the concentration of fucoxanthin was 0.05mg/m L, the scavenging rate of DPPH was 93.6%; the superoxide anion clearance ratio was 77.5%; the clearance ratio of hydroxyl radicals was 84.8% and the deoxidization ability was 2.2. Then the protective effect of fucoxanthin against RAW264.7 murine macrophages oxidative damage was studied, using a oxdative stress model triggered by H2O2(0.5m M, 12h). The livability of cells was measured using MTT. The results showed that addition concentration of fucoxanthin at 20μmol/L after 24 hours significantly(P<0.05) ease the inhibition of macrophages proliferation and increased the level of antioxidase SOD and GSH-Px, and cut down the liberation on NO, LDH. In summary, Fucoxanthin was a type of potent antioxidant. 3. Effect of fucoxanthin on immune response on RAW264.7 cellsRespectively, four models, add fucoxanthin to macrophages alone or under inflammatory conditions and the function of protection and repair on inflammatory cells by fucoxanthin were been processed. Compared control group after fucoxanthin treated cells alone, fucoxanthin could significantly increased(P<0.05) the amount of release of IL-6, IL-10 and TNF-α. And it showed the significant small number than LPS(P<0.05). On conditions of inflammation, the level of IL-6, IL-10 and TNF-αis significantly decreased(P<0.05) by fucoxanthin to ease the inflammation of the cells. Moreover, parallel fucoxanthin with control group showed fucoxanthin had more activities primarily by inhibiting proinflammatory cytokine TNF-α expression or raising the level of anti-inflammatory cytokine IL-10 play an important role in the protection and recovery of inflammatory injury cells.In conclusion, Sargassum horneri is rich in fucoxanthin. The fucoxanthin can be a good health care product with antioxidant and anti-inflammation ability. We hope this paper can be benefical to the further utilization of fucoxanthin.