The Technology Of Preparation And The Study Of Bioavailability Of The Glutamic Acid Chelating Calcium

In view of the present domestic egg shell low value use of practical problems and causepollution to the environment and sotudy，egg shells as calcium source，preparation of calciumglutamate chelate，intended to find a reasonable，effective way to make calcium carbonate andamino acids in egg shell and chelating，provide reference for reuse and eggshell nutrientresources discarded change and provide a new way for the preparation of novel calciumpreparations. The optimization of machining process，analysis of potential advantages，notonly can reduce the egg shell resource waste，and can get more economic benefit. Research ondifferent single factor condition preparation results，determine the experimental factors inresponse to glutamate，the optimum process conditions and parameters optimization ofchelating calcium preparation of egg shell.Calcium glutamate chelate in preparation foridentification， storage stability， and animal experiment， the evaluation of biologicalabsorption and utilization rate and increase the bone density of rat.The main results are as follows：（1）By single factor experiment and response surface design，the effects of technologicalfactors chelating rate according to the order ratio> pH> temperature> time，the optimalpreparation conditions：the ratio of glutamic acid and eggshell powder is3:1，pH is equal to7，the chelating temperature is70℃，chelating time60min. Under the optimal conditions，thechelating rate was63.88±0.15%. By optimizing the parameters of the response surfaceexperiment，solid to liquid ratio of the best：1:20.（2）Ammonia calcium acid chelate preparation for powder，has a special flavor，yellowish，calcium content is9%，the solubility of78.42g/100g in water solution，pH was6.23±0.05，good stability.（3）In normal feeding animals in each group，the high-dose group rat bone mineraldensity，bone calcium content，bone stock weight，bone calcium load higher than the controlgroup and the corresponding high doses of glucose in the control group（P<0.01），high-doseanimal when feeding4wk height of not less than the same level of calcium gluconate group；10wk body weight of each group do not differ significantly. Apparent digestibility of calciumincreased compared with control group，the difference was significant（P<0.01）；apparentdigestibility of calcium and high dose group was96.27±1.55higher than the correspondingdose of calcium gluconate group of4.2%. L-upper femur maximum load/N high，mediumand low dose group were higher than the control group and high-dose calcium gluconategroup14.86%（P<0.01），9.4%，1.03%（P<0.01）.Rat femoral bone mineral density in thelow dose group than in the high-dose group of5.9%glucose，middle and high dose groupwere higher than the control group，the high dose of calcium gluconate11.8%（P<0.01），17.6%（P<0.01）.