Reproductive And Developmental Of Drosophila After Exposure To Carbon Nanomaterials

In this work, we choose Drosophila melanogaster as a model organism to studythe reproductive development of the flies exposed to carbon nanomaterials. Throughoral exposed carbon nanomaterials (single-walled carbon nanotubes SWCNT andmulti-walled carbon nanotubes MWCNT, graphene oxide GO), the research processof laying and pupation shows that carbon nanomaterials has a dose and time effect ondrosophila development and the mechanisms of biological molecular signalingpathways in the process of the laying and pupation.First，carbon nanomaterials were characterized by Imaging，Scanning electronmicroscopy (SEM) and Size and Zeta potential. The results showed that carbonnanomaterials in the water were relatively stable，and it provided the prerequisite forthe experiment.Second，we also researched the different kinds of carbon nanomaterials on the flyreproduction capacity and the influence of survival life. The number of eggs producedby the single-walled carbon nanotubes SWCNT and multi-walled carbon nanotubesMWCNT exposed drosophila were counted. It was found that the number of eggs wasincreased. And graphene oxide GO exposed drosophila were counted. It was foundthat the number of eggs was reduced.Finally, Highly conservative PI3K/AKT/mTOR signaling pathway and moltinghormone and juvenile hormone levels by controlling the Yolk protein expression toregulate the laying of fruit flies. It is worth noting that SWCNTs oral exposed femalelaying eggs more sensitive than males. Further research shows that the drosophilamelanogaster oral exposure SWCNTs by raising the PI3K/AKT/mTOR signalingpathway and alter hormone levels increased the number of eggs. Oral exposure to thestudy of SWCNTs reveals the function of a novel effects in animal embryonicdevelopment and its potential other Suggestions. In conclusion, carbon nanomaterials can induce fruit flies fertility changes, andinfluence the development of fruit flies. In addition，We further study the carbonnanomaterials after exposure of the adult flies in the offspring of the influence of thedevelopment of some specific biological molecular signaling pathways.