Preparation And Properties Of Collagen Peptide Grafted Carboxymethyl Cellulose And Its Derivatives

Carboxymethyl cellulose is a kind of biodegradable, good compatibility,non-toxic, water-soluble cellulose derivatives. Its structure containing carboxyl andhydroxyl groups, the introduction of new groups or compounds can be modified toadd some new properties. This paper study the preparation of collagen peptidegrafting carboxymethyl cellulose and its derivatives. This prepared material has goodantioxidant activity, and it can promotes cell growth and adhesion and it has a goodapplication prospect in wound healing. In this paper, the specific research contentsand results are as follows:1. With EDC (1-(3-dimethyl amino propyl)-3-ethyl carbon imide) and NHS(N-hydroxy succinimide) as cross-linking agent, through the single factor experimentmethod, the different degree of substitution of collagen peptide graft carboxymethylcellulose products has been prepared. Optimization of reaction conditions are: massratio of collagen peptide to carboxymethyl cellulose is1.0/0.6; reaction temperature is55℃; reaction time is20h. The experimental results of antioxidant activity showedthat with the increase of DS and concentration, the scavenging effect of DPPH,hydroxyl and superoxide increased, and reducing power also gradually enhanced.Low molecular weight of products has better ability on scavenging effect, andreducing power decreases with increasing molecular weight. Fibroblast cellsproliferation and adhesion experiments showed that the carboxymethyl cellulosegrafting with collagen peptide can promotes fibroblasts proliferation and adhesion.Cell viability increased with the concentration first increases and then decreases, andincreases with the degree of substitution.2. With carboxymethyl cellulose as raw materials, the sulfating agent (N(SO3Na)3) asesterification agent, sulfate carboxymethyl cellulose was synthesised. Then usingEDC and NHS as crosslinking agent, through the single factor experiment method,different degree of substitution of carboxymethyl cellulose sulfate-collagen peptidehas been made in aqueous solution. Optimum conditions of the reaction are: the massratio of collagen peptide to sulfate carboxymethyl cellulose ratio is0.6/0.3, reactiontemperature is35℃, the reaction time is20h.The experimental results of antioxidantactivity showed that the scavenging effect of DPPH, hydroxyl and superoxide radicals increases with the increase of concentration and DS, and reduction ability alsogradually enhanced. With the reduction of molecular weight, the scavenging effect ofradicals increased. Fibroblast cell proliferation and adhesion experiments showed thatthe cell viability increases with the concentration first increases and then decreases,adhesion rate increases with the concentration, and both of them increased with thedegree of substitution.3. Using carboxymethyl cellulose as raw material, quaternary ammoniumcarboxymethyl cellulose was synthesized with3-chloro-2-hydroxypropyltrimethylammoniumchloride (CHPTAC) as etherifying agent in aqueous solution. With EDCand NHS as crosslinking agent, through the single factor experiment method, thepreparation of the different DS of collagen peptide grafting quaternary ammoniumcarboxymethyl cellulose has been made. The optimal conditions of reaction are: themass ratio of collagen peptide to quaternary ammonium carboxymethyl cellulose is0.6/0.3, reaction temperature is55℃, the reaction timeis24h. The experimentalresults of antioxidant activity showed that with the increase of concentration and DS,the scavenging effect of DPPH, hydroxyl and superoxide radicals increased, andreducing power also gradually enhanced. With the increase of molecular weight,scavenging effect first increases and then decreases, the optimum molecular weight is6.7kDa. Fibroblast cells proliferation and adhesion experiment results showed that thecell viability and adhesion effect decreased with the concentration increases andincreased with the DS increased.