Pollution Stress And HSPs Response Of Lead And Cadmium Combined With Glyphosate On Caenorhabditis Elegans

It is increasing severe that environmental pollution problems caused by organophosphate pesticides and heavy metals. The pollution of the environment often exists more than one contaminate, so the study on the joint toxicity of pesticides and heavy metals has significant meanings. The paper studied the toxicological effect and HSPs response by a typical organophosphate pesticide-glyphosate and two heavy metals-lead and cadmium, using a model organism Caenorhabditis elegans. We selected1/5LC50,1/2LC50and LC50values of glyphosate, Pb and Cd. Thus, for a particular experiment, the concentration of1/5LC50(1/2LC50, LC50) value of glyphosate was mixed with the concentration of1/5LC50values of Cd/Pb, resulting in a final solution of Glyphosate-metal mixture at different concentrations, recording the mortality of C.elegans and its mutants. The other end point choosed head thrash, the number of offspring of C.elegans and its mutants exposed to glyphosate, Pb, Cd, glyphosate+Pb and glyphosate+Cd at their own concentration of1/1000,1/100and1/10LC50values.Our data demonstrated that wild type nematodes showed synergism on Glyphosate-Pb, glyphosate-Cd mixtures stress, while the mutants displayed a complicated data on mixtures stress. The head thrash of wildtype C.elegans was a little higher than that of control exposed on low level concentration of Pb/Cd, while the other treatment groups had significant difference at low level concentration, so did the fertility of five treatment groups. The head thrash of JT6130exposing on contaminates showed obvious decrease compared to control. The fertility of JT6130control group had significant decline compared to wildtype control group, but the effect of stress was not stronger on fertility of JT6130than that on wildtype.The head thrash of BC10067control group was lower than that of wildtype control group, and significant decrease of treatment groups could be detected compared to control. The fertility of BC10067had significant decrease compared to control at low level concentration of Pb and Glyphosate-Pb. No obvious difference could be found exposure on glyphosate, Cd and Glyphosate-Cd at low level concentration compared to control, while the fertility had obvious decline at high level concentration. Significant decrease could be detected between RB2612control group and wildtype control group on head thrash, and the head thrash was similar compared to control at the low level concentration while it had significant difference at the high level concentration. No obvious difference could be found between RB2612control group and wildtype control group on fertility, while significant decline could be detected on fertility at low level concentration of treatment groups.