Preparation Of Soy Protein Isolate Based Interpenetrating Network Polymer Hydrogels And Its Properties

Papers in two parts: the first part belongs to science, namely preparation andproperties of SPI-based interpenetrating network polymer hydrogels and its properties;The second part belongs to the industrial practice, the use of high-speed spinning ofnylon6oligomer components were analyzed.Being acted a kind of excellent biopolymer, protein showed favorablebiocompatible, biodegradable, biostable, low cytotoxic, significant targeting and highdrug loading property. Among the available drug carrier systems, protein-basedcarriers represent promising candidates for efficient carrying drug, and many reportsare focused on these fields. A variety of synthetic and natural polymers have beenstudied as drug carriers. Soy protein isolate (SPI) existing in various soy products isone of the most abundant renewable resources and is receiving burgeoning interest fornon-fond usage due to their low cost, biodegradability, and environmental friendliness.In order to improve and exploit the properties of SPI and PHEMA for drug delivery,the interpenetrating polymer networks (IPN) are utilized instead of synthesizing newtypes of polymers.The structure, thermal stability and hydrogel surface morphology ofcopolymers were characterized using flourier-infrared spectrometry (FT-IR),thermogravimetric analysis (TG) and scanning electron microscope (SEM),respectively.First of all, the type of protein and their modification and application as differentprotein-based drug delivery systems have been reviewed. Firstly, we introduced theclassification methods and main types of protein-based drug delivery systems. Then,the progress of several important protein-based drug carriers were reviewed. Thefollowing protein was always used to prepare drug carrier proteins: albumin, soyprotein isolate, prolamin, alcohol-soluble (zein and gliadin), keratin, collagen, elastin,lipoproteins and metalloproteins. In the end, we pointed out the direction ofdevelopment of protein-based polymer drug carriers.Secondly, a novel drug carrier with simultaneous interpenetrating polymernetworks (IPN) hydrogels based on soy protein isolate (SPI) and poly(β-Hydroxyethylmethacrylate)(PHEMA) is reported. IPN create an opportunity to synthesizeSPI/PHEMA IPN based composites that retain the biocompatibility of SPI and acquirebetter stability and mechanical properties due to the PHEMA component. FT-IR confirms that the IPN hydrogel retains the primary structure of backbone chain of SPIand HEMA. SEM pictures showed that the SPI/PHEMA IPN have an interconnectedporous structure that can also be used as a drug carrier. TG results indicated that thethermal stability of SPI/PHEMA IPN had been significantly improved after addingHEMA. Secondly, SPI/PHEMA IPN swollen properties have been studied, they areintelligent hydrogels with metal cation stimuli response. Lastly, In vitro the drugrelease behavior of SPI/PHEMA IPN was investigated under different environmentsusing model drugs such as Bovine Serum Albumin (BSA) and Rhodamine B (RB).And the release effect of BSA-loaded SPI/PHEMA IPN have controlled deliveryproperties with pH sensitivity, and it is better than that of RB-loaded SPI/PHEMA IPN.The results suggested that such an IPN hydrogel can be exploited as carrier candidatefor drug delivery.Thirdly, a kind of novel drug carrier with simultaneous interpenetrating polymernetworks (IPN) hydrogels based on soy protein isolate (SPI) and poly(methyl acrylicacid)(PMAA) is reported. IPN create an opportunity to synthesize SPI/PMAA basedcomposites that retain the biocompatibility of SPI and acquire better stability andmechanical properties due to introducing the PMAA component. The structure,thermal stability and hydrogel surface morphology of copolymers were characterizedusing FT-IR, TG and SEM, respectively. Secondly, SPI/PMAA IPN swollen propertieshave been studied, they are intelligent hydrogels with metal cation stimuli response.Lastly, drug controlled release properties of SPI/PMAA IPN as drug release carrierswere studied, and the results showed that the SPI/PMAA IPN hydrogels havecontrolled delivery properties with pH sensitivity.Finally, after consulted the literature at home and abroad, and combined with theactual production experiences and researches of polyester and other varieties of slicesome experiences. We analyzed the reason of low polymer produced and its physicalproperties during the Nylon6polymerization process, and then we studied theimportant reasones of the content about oligomer (especially cyclic dimer) in the actualproduction and the damage of the production apparatus of the oligomer. Weestablished a separation and extraction method, and determined the composition for theectract through effective ways.