Preparation And Applications Of Au Coatings Of Solid-phase Microextraction On Metal Fiber

Nowadays, commercial SPME coatings exist some drawbacks include fragilityof fiber support, thermal instability and nonresistance organic solvents, fewer species,high cost and short life time, restrict its further application. Therefore, in our paper wefirstly prepared Au-coating SPME fiber using chemical deposition, which possessesremarkable selectivity and adsorption properties. Besides, the extraction properties ofthe coating were studied and the effects of extraction parameters were investigatedand optimized. Coupled to high performance liquid chromatography with a flamethermionic detector, the optimized SPME method was applied to the analysis oforganic pollutants in real water samples. The contents and results will bedemonstrated as follows:In Chapter1, we have summarized and described a brief introduction to SPMEtechnique, including its development, theories, influent factors, types of extractioncoating and related to preparation technology and application fields. The research aimand significance of the study were also discussed.In Chapter2, a robust Au-coated solid-phase microextraction (SPME) fiber wasrapidly prepared on the etched stainless steel wire based on chemical deposition. Thistechnique was proved to be simple and reproducible. The prepared Au-coating wasuniform and dense, and possessed advantages such as resistant to organic solvents,high mechanical strength and unbreakable. In addition, the novel fiber is conductive．In Chapter3, the prepared Au-coating fiber was applied to the determination of5PAHs, i.e., naphthalene, phenanthrene, anthracene, fluoranthene and pyrene inaqueous samples by SAME-HPLC-UV method． The results showed that theAu-coating fiber was effective in extracting PAHs in aqueous sample. A durability ofmore than150times analyses was achieved for one unique fiber. Under optimizedconditions, the calibration graphs were linear in the range of1.00-500and0.20-500μg·L-1and limits of detection were between0.016-0.22μg·L-1(S/N=3). The analyticalresults of real water samples showed that the recovery ranged from86.0%to112.9%with relative standard deviation from2.03%to11.7%. The fiber coating is sensitive,accurate and suitable for the preconcentration and determination of PAHs in real water samples.In Chapter4, in order to investigate different chemical deposition time forAu-coating enrichment and separation capability, we select B[a]p as a typical targetanalytes to further study. Optimize the experimental conditions for the impact of theextraction efficiency and establish real water samples analysis method of B[a]p withSPME-HPLC-UV. Besides, the results were satisfactory.In Chapter5, complete the second self-assembly for Au-coating with1,8-octanediol and investigate its extraction efficiency with BP-3, EHMC, OD-PABAand EHS4UV-filters. In the study, the used SPME fiber can improve thereproducibility of the analysis method and the service life of the fibers. Experimentalconditions affecting SPME such as extraction and desorption time, extractiontemperature, stirring rate and ionic strength were investigated and optimized. Themethod was successfully applied to determination of UV-filters in real water samples.