Protective Effects Of Capillaris On Intestinal Mucosal Barrier In Rats With Alcoholic Liver Injury

Objective: To study the effects of Aplysin on serum TNF-α 、FABP2、TGF-β、D-LA、DAO concentration and FOXO4 expression in intestine in rats of alcohol-induced hepatic injury.Methods: 1. Experimental animals Grouping and model building: Male Wistar rats were randomly divided into the following four groups: control group, normal diet with normal saline, ethanol-treated group, normal diet with ethanol administration,and low- and high-dose Aplysin[ 100, and 150 mg/(kg?bw?d)]plus ethanol treatment groups. Excluding the rats in the normal control group, the other animals were initially received intragastric administration with 56%(v/v) ethanol 5.5 m L kg-1day-1 for the first week, 7.5 m L kg-1day-1 for the second week, 8 m L kg-1day-1 for the third week, 9 m L kg-1day-1 for the fourth week, and 11 m L kg-1day-1for the rest of the 4 weeks. Twelve hours after the last ethanol treatment, the rats were anesthetized, and blood was collected, the small intestine was obtained. 2. the small intestine used for ultrastructural observation by tansmission electron microscope. 3. plasma FABP2 /TGF-β/TNF-α/D-LA/DAO concentration was measured by enzyme linked immunosorbent assay. 4. The expression of FOXO4 in small intestine was detected by immunohistochemistry.Results: 1. In the ethanol-treated group, the ultrastructural changes of small intestine tissue by tansmission electron microscope showed that the intestinal villi fell away, and loosely arranged, tight junction were swelling, however, the control group did not show abnormal ultrastructural. In each dose Aplysin, ultrastructural changes were better than the ethanol-treated group. 2. In control group, the plasma concentration of D-LA and DAO were(4.78±0.67)u mol/L 、(66.65±9.07)U/L, the ethanol-treated group were(6.44±1.83) u mol/L 、(87.87±20.14) U/L, it was significantly elevated compared with control group. the plasma concentration of D-LA and DAO in alcohol+ Aplysin(100mg/kg) group were(5.44±1.23) u mol/L 、(69.47±7.14) U/L, alcohol+ Aplysin(150mg/kg) group were(5.06±1.32) u mol/L、(67.92±7.68) U/L, they were significantly reduced compared with the ethanol-treated group. 3. In control group, the plasma concentration of FABP2, TNF-α and TGF-β were(2.79±1.18)ng/ml,(30.42±18.89)pg/ml and(38.67±19.22)pg/ml, the ethanol-treated group were(3.87±1.01)ng/ml,(59.31±35.01)pg/ml and(62.28±9.33)pg/ml, it was significantly elevated compared with control group. the plasma concentration of FABP2, TNF-α and TGF-β in alcohol+Aplysin(100mg/kg) group were(2.87±1.60)ng/ml,(36.32±19.58)pg/ml and(42.09±19.60)pg/ml, alcohol+ Aplysin(150mg/kg) group were(2.25±1.06)ng/ml,(32.50±18.70)pg/ml and(33.91±22.54)pg/ml, they were significantly reduced compared with the ethanol-treated group. 4. The FOXO4 expression in ethanol-treated group was decreased with comparison of control group, OD value in ethanol-treated group and control group were(0.115±0.064) and(0.264±0.023); compared with the ethanol-treated group, in each dose Aplysin the expression of FOXO4 were increased(P<0.05), alcohol+aplysin(100 and 150mg/kg) group were(0.201±0.089)、(0.231±0.092).Conclusion: 1.The results of ultrastructural changes in small intestine tissue and the plasma concentration of D-LA and DAO revealed that there have intestinal barrier injury in rats which with alcohol-induced hepatic injury. 2. Aplysin can improve intestinal barrier damage caused by alcohol exposure, and its mechanism may be related to aplysin regulates the expression of protein and reduces the release of inflammatory factors.