Study On Serum Medicinal Chemistry Of Active Components Of Hypnotics From Chicory

BackgroundThis study is parts of the national natural foundation,which also was supported by Beijing University of Traditional Chinese Medicine (2014-JYBZZ-XS-092).With the rapid development of economic level, residents increasing material and cultural level, the daily diet changes。People high-energy food intake increased, resulting in an increased incidence of hyperuricemia, the elderly prone disease, there is the development trend towards younger age. While the incidence of hyperuricemia in the same time will be accompanied by obesity, diabetes, coronary heart disease, hypertension and other related diseases, metabolic diseases, has become one problem troubled people.Chicory, also known as endive, coffee grass, French endive, etc., native to the Mediterranean, Central Asia、North Africa, planting a long history. Chicory is often used as a vegetable in Europe. Chicory can be used as animal forage, raw sugar and coffee substitutes. Xinjiang, Shandong, Inner Mongolia, have grown the chicory. Chicory more applications mainly in Xinjiang, Mongolia and other regions, is Cichorium glandulosum Boiss.et Huet and Cichorium intybus L. Dry ground and root. It can improve the appetite, increase digestion and treat liver diseases, etc. Primarily for the treatment of heat jaundice, stomach eat less, edema, oliguria. The function of reducing animal uric acid, glucose, triglycerides and other pharmacological effects. Chicory flower extracts to animals after injection can enhance central nervous excitement and heart activity, root extract can increase appetite and promote digestion, The infusion of high concentrations may be enhance gastric secretion. Chicory seeds containing non-specific phytohemagglutinin. For the mentioned reasons, chicory has a bright prospect in future. However, the chicory’s mechanism is not perfect, material basis of efficacy is not yet clear. Chicory Extract although has a clear pharmacological effects.But the target component is too vague, unknown targets, for clinical use chicory caused great difficulties.Traditional Chinese medicine research methods is separation and extraction, focusing on the chemical composition of the extraction activity in vitro conditions, the extracted compounds may be effective substance ingredients of traditional Chinese medicine. According to chemical drug research model on Chinese medicine, can’t reflect the characteristics and advantages of Chinese medicine treatment of disease. The Chinese medicine pays attention to the overall concept of the patient as a whole, to apply traditional Chinese material medica. Serum pharmacochemistry of traditional Chinese medicine is the identification of serum drug absorbed into the blood after oral medicine ingredients, researching on changes which ingredients will be absorbed into the bloodstream after metabolic, These absorbed into the bloodstream of drug ingredients that can comprehensively reflect the overall characteristics of traditional Chinese medicine, may be potential efficacy of herbal substances Through modern frontier test technology, analysing for pharmaceutical ingredients. Therefore, analysis by the oral pharmaceutical ingredient contained in the serum, identified that materials play a direct role in vivo, will be an effective way to quickly and accurately determine the efficacy of the material basis of traditional Chinese medicine., promoting the development and promotion of traditional Chinese medicine.The purpose of this experiment is study Chicory’s aboveground serum pharmacochemistry, ensure that all aspects of research on traditional Chinese medicine applications, clear chicory aboveground herbs medicinal ingredient, Chicory in the treatment of clinical disease has important practical significance.Research ContentThis study includes theoretical and experimental research in two parts.Theoretical research1. The research progress of serum pharmacochemistry of traditional Chinese medicineThis part introduce the birth of the Serum pharmacochemistry of traditional Chinese medicine, research methods、serum preparation、detection means、prototype components、 the metabolite absorbed into the blood, thoughts the literature review on serum pharmacochemistry outlooks were combingExperimental Research1 First, analysis the chicory component of the aerial parts by high performance liquid chromatography-tandem mass spectrometry, clear the extract ingredients, Lay the foundation for future in vivo analysis of chicory. By explicitly chicory component sources, combined Chicory serum chemistry study with analysis the metabolism in rats, in order to ensure chicory aboveground medicinal ingredient. This study uses the methods of Serum pharmacochemistry of traditional Chinese medicine, the medicinal chemical ingredients which are absorbed into the bloodstream。The ingredients are absorbed into the bloodstream, maybe be the efficacy of substances. While collecting excrement such as urine and feces of rats, some ingredients are likely to be intestinal flora catabolism, in the form of metabolites excreted. From the blood components and excretion, looking chicory metabolic components in rats, lied the foundation for the clinical application of chicory.1. Qualitative analysis of the main part of the ground chicory component First prepared chicory extract of aerial part, liquid chromatography-mass spectrometry technique chicory comprehensive analysis of the aerial parts of herbs, get most of the chemical composition of the secondary and tertiary structure of mass Information, for future experimental component analysis.2.The chicory’s metabolic analysis in vivo studies in normal ratsThis section contains two experiments:The Chicory constituents were absorbed into blood of in the normal state rats and the metabolism studies in a normal state rats’urine and feces.Under normal conditions of farm animals, administration animals fed chicory extract three days, using high-performance liquid chromatography liquid MS analysis the blood of normal and administrated, clearly the peak of the blood component. By comparing the standards of conduct identified the differences peaks.After collecting chicory rats administered under normal conditions of urine and feces of rats, using the same means and methods with blood, find out the chicory’s constituents in urine and feces.3. Analysis of in vivo metabolism of chicory in pathology in ratsThis section contains two experiments:Chicory constituents were absorbed into the blood in rats with hyperuricemia and chicory in hyperuricemia model rats urine, feces metabolism studies. Due to the absorption of animal drugs under pathological conditions there may be different absorption and normal animals, the drug were absorbed in a state of the Pathological animal., which ingredients are absorbed in play at the same time into the body effect. By comparing the normal control group, the pathological control group, the treatment group chicory plasma profiles, Observed the chicory absorbed into the bloodstream after administration of therapeutic ingredient.By collecting hyperuricemia rat urine and feces after treatment, using the same means and methods with blood, find out the chicory’s constituents in urine and feces.Results1. First prepare the portion of chicory extract, drinking high-fructose shape hyperuricemia pathology in rats, treating the rat used the chicory’s extract, and observing levels of uric acid index, disease characteristics clearly determined the efficacy of chicory.2. By comparing the normal status animal control serum and chicory serum fingerprints, found three components appear in plasma administration. By comparing the chicory extract ingredients list, there is no prototype ingredients are absorbed into the blood. There may be a pharmaceutical ingredient in the process of metabolism occurs in vivo absorption. Normal state administration in the urine of rats found 13 chemical compositions. Detected nine components, the lactucin which prototype component elements in rat feces.3. By shaping successfully of high uric acid pathological model rats, Chicory’s extract has a clearly effect of treating the uric acid. By comparing the normal group, model group plasma, In the treatment group plasma, no chicory extract pharmaceutical ingredients are absorbed into the plasma. In the treated urine, we also discovered the prototype components such as the lactucin compound.