Pharmacological Study And Chemical Constituents

This thesis includes two parts:Part I:literature review.In this part, the pharmacognosy identification、botanic origins、chemical constituents and pharmacology of Spiranthes sinensis were summarized. The advances in cellular models of insulin resistance and the relationship between insulin resistance and diabetes were also introduced. The review included research progress of wound healing in diabetic animals as well.Part Ⅱ:Experiments. This part can be divided into six aspects:1 The cytotoxicity assay in vitro of 70% ethanol extract from Spiranthes sinensis. In the experiment the human hepatoma cells (Hep-G2) and the normal human liver cells(LO2) were used as the tested cells. The resμlt showed that the tested drug could promote the proliferation of the tested cells.2 The insulin resistance test of 70% ethanol extract from Spiranthes sinensis. The insulin resistance cell model was induced by high concentrations of insulin. The result indicated that the tested drμg could reverse resistance to the insulin resistance cell model.3 Observe the effects of 70% ethanol extract from Spiranthes sinensis on the blood glucose of diabetic mice. ICR male mice were intraperitoneally injected alloxan. The tested drug were continuously given by intragastric administration to the tested mice. The result showed that the tested drug could improve diabetes hyperglycemia and stabilize their blood glucose levels.4 Observe the effects of 70% ethanol extract from Spiranthes sinensis on the wound healing of diabetic mice. The tested mice model were wounded in the back of diabetic mice. The tested drug were continμoμsly given by intragastric administration to the tested mice.The rate of wound healing indicated that the tested drμg could promote wound healing of diabetic mice.570% ethanol extract from Spiranthes sinensis were divided into 4 parts by macroporous resin. The four parts included 20%、40%、60%、80%ethanol-water elusion positions. And the total flavonoids in the four parts were determined respectively by MV spectrophotometry. The result was that the total flavonoids were most in the 40% ethanol-water elusion position.6 Kaempferol-3-O-sambubioside and Spiranthes sinensis flavonoid A were isolated from Spiranthes sinensis at the first time. The structure of the compounds were identified by MS、IR spectroscopy and NMR spectrometer.