Expression And Significance Of TRAIL Receptor Dr5, Dcr3, And CASPASE-3 In Bladder Cancer And Precancerous Lesions

Background and Objective:the incidence of bladder cancer tumors in the urinary system accounted for the first one in china . Clinically, the treatment of bladder cancer based on surgery, Including bladder tumors resection and open surgery, While giving the postoperative adjuvant chemotherapy and immune therapy. Although it had good clinical results, But the recurrence rate remained high. Expression of DR5, DcR3, and caspase-3 in bladder cancer, bladder papilloma and normal bladder mucosa were detected by immunohistochemical SP method and RT-PCR,In our Experiment,DR5, DcR3, and caspase-3 might contribute to the occurrence and development of bladder cancer,which provided new ideas to treat of bladder cancer.Methods:Expression of DR5, DcR3, and caspase-3 in 30 cases of bladder cancer, 20 cases of bladder papilloma and 15 cases of normal bladder mucosa were detected by immunohistochemical SP method and RT-PCR .Results:Positive expression rate of DR5 in normal bladder mucosa, papilloma, bladder cancer were 70%, 45% and 30%,repectively,which showing a decreasing trend; Positive expression rate of DcR3 in normal bladder mucosa, papilloma, bladder cancer were 20%, 37% and 88%, repectively,which showing a increasing trend; Positive expression rate of caspase-3 in normal bladder mucosa, papilloma, bladder cancer were 86%, 65% and 22%,repectively,which showing a decreasing trend; Quantitative analysis of DR5mRNA, DcR3 mRNA and caspase-3 mRNA further support the results of immunohistochemistry.Conclusion:DR5, DcR3, and caspase-3 might contribute to the occurrence and development of bladder cancer. The test of DR5, DcR3, and caspase-3's expression may have a certain value for the early diagnosis of bladder cancer.