The Mechanism Of Antioxidant In Vitro And Anti-inflammatory About Chitosan Oligosaccharide And Geniposide

Chinese herbal is rich in resources, low cost, easy to use and has little toxic and side-effect. It's very meaningful to research its antioxidant and anti-inflammatory role systematically. Chitosan oligosaccharide (COS) is derived from crab shell and Good water-soluble, it's safety and easy to be absorbed and is widely used as food additives .Gardenia is for both food and traditional Chinese medicine in China, Geniposide is the most abundant in Gardenia and plays an important role in the medicinal effects of Gardenia. This topic has chosen the two Chinese herbal to find out their anti-inflammatory and antioxidant effects.Microglia are uniform distribution in central nervous system, activated microglia produce reactive oxygen species, leading to nerve damage and degeneration, Alzheimer's disease (Alzheimer's disease, AD) and Parkinson's disease (Parkinson's disease, PD) and other neurodegenerative diseases are closely related with the high oxidative stress. Human umbilical vein endothelial cells (HUVEC) are similar biological characteristics with artery in many ways, are commonly used in the study of atherosclerosis (AS) and other diseases.This issue proved the antioxidant effect of COS in vitro and further studied in the N9 microglial cells on the antioxidant. We found that chitosan can reduce the induced N9 microglial cells from oxidative injury that caused by lipopolysaccharide (LPS). In the second part of this issue we made HUVEC stimulated by LPS, leading to intercellular adhesion molecule -1 (ICAM-1) increased. HUVEC pretreated with Geniposide resistant to LPS stimulation, reduced ICAM-1, resulting in inflammation .