The Clinical Analysis Of Risk Factors Forhbv Reinfection After Liver Transplantation Of HBV Related Disease

Objective Through clinical data and experimentation data detect To investigate the risk factor of hbv rein - fection after liver transpl -antation.Methods 83case of liver transplantation in our hospital and Zhejiang University School of Medicine . Clinical data: (1) Quantitative PCR Detection of preoperative serum HBV DNA (+) the number of cases, (2) sex, (3) age, (4) the number of patients with hepatocellular carcinoma, (5) re-infection of hepatitis B the number of cases. Experimental tests: (1) collecting the selected elbow vein after liver transplantation cases (10ml), (2) isolated from peripheral blood mononuclear cells (Ficoll density gradient centrifugation), (3) fluorescence quantitative PCR detection after liver transplantation peripheral blood mononuclear cells HBVcccDNA (+) the number of cases. The above data through logistic regression analysis, search for the risk factors of HBV reinfection after liver transplantation.Result Retrospective analysis of clinical data: (1) combined with liver cancer is 31case; (2) male is 43 cases; (3) age is 44.33±7.32 years (4) bleeding is 1414.46±1082.35ml (5) before liver transplantation, serum HBV DNA (+) is 19 cases; (6) HBV re-infection is 41 cases; (7) HBV recurrence is 8 cases; (8), 4 patients died . assay: PBMC-HBVcccDNA (+) is 21cases. logistic regression analysis (P value, OR value). Serum HBV cccDNA Preoperative (P,0.002,OR,15.582), PBMC- HBV- cccDNA(+) postoperative (P,0.011,OR,7.591), affiliate hepatoma(P,0.022,OR,5.298), blood loss of operation(P,0.002,OR,1.001),sex(P,0.323,OR,0.533),age(P,0.664,OR,1.012)Conclusion Age and sex was not relation of HBV reinfection after liver transp lant- ation. Blood loss of operation, affiliate hepatoma, Serum HBVcccDNA Preoperative and PBMC HBVcccDNA(+)postoperative was risk factor of HBV reinfection after liver transplantation.