The Abstraction And Identity Of [11C]MP4A-a PET Imaging Agent For Brain Acetylcholinesterase Activity Made By Ourselves

Objective: To improve and optimize the methods for the separation, purification and identification of self-synthetic [11C]MP4A in crude and to develop a method for the determination of radiopharmaceutical [11C]MP4A. Methods : An extended polar semi-preparation column was applied to the separation and purification of radioactive crude [11C]MP4A. The mobile phase consisted simply of a binary mixture of ammonium acetate with ethanol. The purities in chemistry and in radiochemistry of [11C]MP4A after one-step purification were confirmed by a combination method of ultra-violet spectrophotometry in tandem with electrochemical detection and isotopic determination, respectively. Results: Using the improved method, the retention time of the precursor and the [11C]MP4A were, in minutes, 2.2～2.8 and 8.5～9.0, respectively. The purity for collected fraction of [11C]MP4A was over 99% in chemistry and was 100% in radiochemistry, respectively. Conclusions: The radio-labeling [11C]MP4A with high purity in both chemistry and radiochemistry can be harvested with presented method through only one-step purification that is in favor of yield of imaging agent product and shortening the time of operator exposed to radiation.