Research On The Protective Effects And Mechanism Of Aiweixin Liquid In MIRI

Objective: To explore the effect of Aiweixin on PMN, NO and H2S when the rats suffered from myocardial ischemia-reperfusion injury. Methods: forty Wistar male rats, weighting (200～250)g, were randomly divided into four groups, which were sham- operated group, ischemia reperfusion injury (I/R) group, pretreatment group of Aiweixin and treatment group after Aiweixin. There were ten rats in each group. The model of MIRI was produced. The process of ECG was recorded to measure and analyze the change of T wave by using BL-420 biological signal acquisition system. Measuring the activity change of CK, SOD, MPO, NOS and the concentrations change of MDA and H2S in rat's blood plasma. Measuring the activity change of CK,SOD,MPO,NOS,CSE and the concentrations change of MDA and H2S in the rat's cardiac muscle. Observing the morphological change of rat's cardiac muscle. Results: 1)To compare the ischemia-reperfusion injury (I/R) group, in pretreatment group of Aiweixin and treatment group after Aiweixin, elevation of T wave was lighten, in blood plasma and cardiac muscle, activity of CK, MPO, iNOS and the concentrations of MDA was decrease, and activity of SOD was improved; 2) To compare pretreatment group of Aiweixin, the increase of concentrations of MDA and decrease activity of SOD was more conspicuous in treatment group after Aiweixin. 3) The concentrations of H2S was increased in the blood plasma of pretreatment group of Aiweixin. The concentrations of H2S and the activity of CSE was decrease in cardiac muscle of treatment group after Aiweixin. Conclusion: Aiweixin can refrain membrane lipid peroxidation of the model of MIRI whose mechanism is possibly concerned with advancing the activity of SOD and decreasing the concentrations of MDA and refraining the activity of iNOS and WBC'S aggregation.