The Study Of Human Papillomavirus (HPV) Type 16 E6 Gene Testing In Urine From Females With Cervical Lesions

Backgrund:Infection of specific types of high-risk human papillomaviruses (HPVs) causes cervical cancer in women. Conventional test for genital HPV infection requires collection of scraped cervical cells or biopsy specimens, which involves invasive procedures. Utility of non-invasive urine sampling for detection of HPV in women and their male sexual partners is controversial. The validation of this urine-based HPV DNA test is of immense value not only in screening large population and children but also for HPV vaccine monitoring in adolescents. objective:To investigate clinical value of HPV16E6 gene detection in urine samples from females with cervical lesions. Methods:Paired self-collected urine and cervical scrapes were collected from 112 females with cervical lesions or HPV infections,including 33 specimens with invasive carcinoma(ICC), 29 specimens with high grade cervical intraepithelial lesion(CINII-III ),15 specimens with low grade cervical intraepithelial lesion (CIN I ),35 specimens with cervicitis.Urine and cervical samples were also collected from 20 healthy women (as control group). HPV 16E6 gene PCR was performed on these specimens. The total positive rate of HPV16E6 gene in urine samples was 27.7%, 2.9% with cervicitis, 13.3% with CIN I, 41.4% with CIN II-III and 48.5% with ICC. There were statistic differences in four groups (P<0.05). The total positive rate of HPV16E6 gene in cervical scrapes was 48.2%, cervicitis, CINI , CINII-III and ICC was 17.1%, 40% , 65.5% and 69.7% respectively. The accordant positive rate of HPV16E6 in urine and cervical samples was 57.4% (31/54) , and KI was 0.64.There was statistic difference between two kind of samples for HPV16 E6 gene detection (P<0.05). HPV16E6 gene was not detected in any samples from 20 healthy women. Conclusions:HPV16E6 gene is the principle risk factor associated with cervical cancer. Urine samples have some accordance to cervical scrapes for HPV16E6 detection in females with cervical cancer and its precancerous lesions, but it can not be routinely used as medium for high risk HPV detection. It is propably a promising method for special population (such as adolescents) to detect high risk HPV.