Studies Of The Protective Effect Of Edaravone In The Acute Phase Of Radiation-Induced Brain Injury

Objective:To illustrate the neuroprotective effect of edaravone on the radiation-induced acute brain injury and to explore the possible mechanism.Methods:54 adult New Zealand white rabbits with no sex limited, weighing 2.2-2.5 kg were randomly divided into 3 groups:blank control group[shame-radiation combined with normal saline (NS)];experimental control group [radiation combined with normal saline (NS)] and experimental group [radiation combined with MCI-186 (edaravone)]; Each group has 18 rabbits. Each group was further divided into 3 subgroups according to different phase. The half brain of New Zealand rabbits were irradiated with a single dose of 30GY using 60Co so as to establish the radiation-induced brain injury model. sacrificed them successively and six brain tissue samples were obtained at at the 24h,3d,7d after irradiated in each group. observing target lesions grossly;The light microscope histological changes of rabbits'brain's were observed after hematoxylin-eosin (HE) staining; Immunohistochemistry was used to detect the expression of NF-κB p65 in the irradiated brain injury tissue, Get 0.5g tissue of brain cortex to mensurate the degree of containing water or moisture in determining the degree of dropsy in brain; malondialdehyde (MDA) were measured by the absorption spectrometry. One way-ANOVA was used for analysis.Result:1.Experimental rabbits brain tissues in the experimental control group which received 30GY radiated occurred clear histopathologic changes; In the experimental group, brain tissues also occurred changes, However, compared with experimental control group,the degree of injury is slighter significantly at 3d after irradiated.2.The expression levels of NF-κBp65 protein in experimental control group at all time points were higher than those in blank control group (P<0.05). The expression levels of NF-icBp65 protein in edaravone group had a result of lower NF-κBp65 protein at 3d and 7d after irradiated than experimental control group significantly.(P<0.05).3. MAD in brain tissues after irradiation raised significantly as well as the water content in brain tissues; the application of Edaravone decreased the MDA level and alleviated brain edema.Conclusion: 1. The half brain of New Zealand white rabbits were irradiated with a single dose of 30GY using 60Co which can cause histopathologic changes in brain tissue and establish the radiation-induced brain injury model.2. The expression of NF-κBp65 protein was increased in the acute stage of brain injury induced by radiation. Edaravone could effectively relieve the inflammatory reaction in the acute stage of brain injury after irradiated by inhibiting the activation of NF-κB.3. In rabbit brain tissue of acute radiation-induced injury, water and MDA content emerge change. Edaravone can both inhibit the reaction of lipid peroxidation and alleviated brain tisue edema which might protected brains from radiation injury.