The Impact Of Repaying Of Rat Carotid Artery In VSMC Migration After PTA And In The Expression Of Integrin β3,MMP2 And FAK

Objective: To investigate the migration of VSMC and expression ofβ3 integrin,MMP2 and FAK of rat carotid artery after PTA treated in rapamycin.Methods: Production of rat model of carotid artery after PTA,which was randomly divided into 2groups: Model group (After modeling, A normal diet, freedom of movement) and Rapamycin group(After modeling, Rapamycin 2mg/kg/d to be fed, A normal diet, freedom of movement),Using morphology, immunohistochemistry and RT-PCR to detect the vascular stenosis rate, VSMC migration and the change of expression ofβ3 integrin, MMP2, FAK in each group.Results: (1)Morphological observation shows that: on the point of 7 days and 28 days ,intima and media thickness of rapamycin group is less than the same point in time of the model group (P<0.05), the loss of lumen area and the rate of vascular stenosis of rapamycin Group was lower than the model group on the same time point (P<0.05);(2) VSMC exists migration after PTA, on the point of 7 days and28 days ,after the intervention of rapamycin, VSMC migration of rapamycin group is lower than the model group at the same time points.(Expression of endometriumα-smooth muscle actin at 7 days, 28 days of P<0.05);(3) Detect the expression ofβ3 integrin, MMP2, FAK by immunohistochemistry ,we observed that on the 3 days, 7 days, 28 days time point , MMP2 expression of rapamycin group was lower than the model group on the same time points, (P<0.05);on the 7 days, 28 days time pointβ3 integrin, FAK expression of rapamycin group were lower than the model group on the same time points, (P <0.05);(4) Detect the expression of FAKmRNA, MMP2mRNA of each group by RT-PCR, we observed that on the 7 days, 28 days time point, FAKmRNA expression of rapamycin group was lower than the model group on the same time points, (P<0.05); on the 3 days, 7 days, 28 days time point, FAKmRNA expression of rapamycin group were lower than the model group on the same time points, (P<0.05).Conclusion: Rapamycin can inhibit the VSMC migration, reduce neointimal proliferation, while reducing the rate of vascular stenosis, and have a negative impact on the expression ofβ3 integrin, MMP2, FAK, this play a role in inhibition of the VSMC migration and proliferation.