HPLC Method Of Asarum Medicated Serum Methyl Eugenol And Aristolochic Acid A's Content Determine

PurposeUsing high performance liquid chromatography (HPLC) of asarum medicated serum methyl eugenol and the aristolochinc acid A (AAⅠ) content determination, designed for clinical safely and effectively use asarum and objective evaluation, and to provide the evidence asarum establish serum containing drugs were asarum methyl eugenol and AA I content of HPLC method.Methods1. The serum containing medicine preparation of asarumRoots in north asarum in drying oven is heating temperature of 40 degrees Celsius (1h), and then the ball grinding mill grind crushing, screening (5), asking 80 mesh shade, powder asarum closed. Set aside. Adult SD rats will be divided into two groups at random, namely the blank serum containing medicines and serum group. One group of serum containing asarum drug dosage asarum for 1.78 g/kg, Given the amount of blank serum group saline.2 times every day for medicine, eight days. In the final after dosing 2h shows in rats orbit. Blood will gather at room temperature for 30min after supernatant 20min centrifugal separation,3000rpm, serum, serum 2ml, add methanol 8ml, blending,2500rpm 10min centrifugal and supernatant 65℃, baths, swing to methanol concentration, treatment muon m microfiltration membrane filtration aseptic, packing,-20℃.best preservation conditions.2. HPLC in serum containing medicine asarum content determination of methyl eugenol.By HPLC, high performance liquid chromatographic instrument for dianne:P680 type feed pump, online dehydrator, manual sampler, TCC-100 oven, PDA-100 photodiode array detector, chromeleon chromatogram workstation. Conditions:Diamon TMODS-C18 column for 250mm x 4.6mm,5μm, Mobile phase for methanol-water-0.025 mol/l phosphate (78:21:1); Column temperature for room temperature, For 250nm detected wavelength, For lml/min, velocity The 20μl, determination of asarum medicated serum methyl eugenol content.3. HPLC serum containing medicines for asarum AAⅠcontent determination.By HPLC, high performance liquid chromatographic instrument for dianne:P680 type feed pump, online dehydrator, manual sampler, TCC-100 oven, PDA-100 photodiode array detector, chromeleon chromatogram workstation. Conditions:Diamon TMODS-C18 column for 250mm x 4.6mm,5μm,Mobile phase for methanol-water-acetate (72:27:1); it:Column temperature for room temperature, For 390nm detected wavelength, For lml/min, velocity The 20μl, determination of asarum serum containing drugs AAⅠ.ResultsAsarum serum containing medicine for the content of methyl eugenol 0.0032 mg/ml. Asarum serum containing drugs AA I levels below the detection limit, can be ignored.Conclusion1. Preliminary conducted HPLC serum containing medicine of asarum methyl eugenol and content determination of AAⅠ; 2. The determination of asarum medicated serum methyl eugenol and AAⅠby HPLC method.3. Asarum medicated containing a certain blood serum concentrations of methyl eugenol, excluding AAⅠ...