| With the enhancement of nation power and worldwide influence, pepople'living standard raises greatly in the 21st century. A series of health problems follow, which should be paid attention to. Diabetes mellitus, which was known as"rich man's disease"in the past, takes on a tendency of universality, civilianful and youthful because of irrationality of diet structure and influence of environment now. Diabetes mellitus which is charactered as hyperglycosemia, is a disease of metabolic disturbance of carbohydrate, fat and protein due to insulin secretion absolutely insufficient or relatively insufficient. Prophylaxis and treatment of diabetes mellitus are the most important and duty-bound responsibility for medical workers owing to the high morbidity rate and death rate of diabetes for the past few years in our country. It is urgent to discover and develop traditional Chinese medicine (TCM) at the time of TCM making for redevelopment.Shuangqing is made up of seven kinds of traditional Chinese medicines. They are Semen Cassiae, Rhizoma Polygonati, Folium Mori, Flos Chrysanthemi, Radix Pueriae, Rhizoma Dioscoreae and Rhizoma Phragmitis, respectively. Folium Mori, Flos Chrysanthemi and Rhizoma Phragmitis have effect of decreasing blood glucose in diabetic rats, which has been proved by experiment. They are also essential components of many kinds of Chinese medicine formulae. It's possible that Shuangqing could decrease blood glucose and complications of diabetic rats in view of above.The present study is undertaken to observe if Shuangqing possesses the effect of lowering blood glucose and protective effect on nerve of diabetic rats induced by intraperitoneal injection with STZ. The concrete experiments are as follows:Part 1 Effect of Shuangqing on blood glucose in diabetic rats Objective: To study the effects of Shuangqing on blood glucose and urine volumn of diabetic rats.Methods: Healthy male Wistar rats (250±20g) were selected. 12 Rats were left as normal control group, and the others were made diabetic model. Rats were treated by intraperitoneal injection with STZ-citric acid balance solution 50mg/kg 12 hours after fasting food but non-fasting water, while the rats in the normal control group were treated by intraperitoneal injection with citric acid balance solution by the same volume. Blood samples were obtained from fossa orbitalis vein of rats 3 days after STZ was given. Hol-automatic biochemistry analyzer was used to determine fasting plasma glucose (FPG). The rats whose blood glucose was higher than 11.1mmol/L were selected, and then randomly divided into 5 groups according to FPG and body weight: (1) Positive control group (glibenclamide, 1mg/kg); (2) High dose of Shuangqing group (4g/kg); (3) Medium dose of Shuangqing group (2g/kg); (4) Low dose of Shuangqing group (1g/kg); (5) Model group. Rats above were treated by intragastric administration with 1ml/100g at 8:30-10:00 everyday, while the ones in normal control group were treated with distilled water by the same volume. The body weight of rats should be registered every 2 weeks for each group, and blood glucose was measured by Hol-automatic biochemistry analyzer 8 weeks after drugs were given.Results:1 Rats in normal control group were active and the fur was smooth and glossy, while the rats in model group were depressed.2 Body weight of rats in normal control group was 346±59g, which was higher than that of rats in model group 205±23g (P<0.05). The body weight of rats in positive control group was 313±93g, which was much higher than that of rats in model group (P<0.05). SQ depressed the loss of body weight gain in a dose-dependent manner. In particular, 2 and 4g/kg of Shuangqing restored body weight gain (P<0.05). SQ 1g/kg had no significant effect (P>0.05) on body weight comparing with model group.3 The urine volume of rats was 78±18ml during 24h in model group, which was much more than that in normal group (P<0.05). In the groups treated with SQ 2g/kg and 4g/kg, the urine volume was much less than that in model group (P<0.05).4 The blood glucose in model group was 31.5±8.4 mmol/L, which was higher (P<0.05) than that (5.9±1.6mmol/L) in normal group. The blood glucose in positive control group was 16.6±7.9 mmol/L, which was obviously lower (P<0.05) than that in model group. The blood glucose in SQ 4g/kg, SQ 2g/kg and SQ 1g/kg was 12.2±7.8 mmol/L, 20.7±11.0 mmol/L and 21.5±10.9 mmol/L, respectively, which was lower than that in model group (P<0.05). Conclusion: SQ 2-4g/kg can increase the body weight and decrease the urine volume of diabetic rats, and SQ 1-4g/kg can decrease the blood glucose of diabetic rats.Part 2 Neuro-protective effect of Shuangqing in DPN ratsObjective: To study the protective effect of Shuangqing on the nerve in DPN rats by measuring nervus coccygeus conducting velocity (NCCV) and thermalgia threshold.Methods: 80 Healthy male Wistar rats (250±20g) were selected. Twelve rats were left as normal control group, and the others were made diabetic model. Rats were treated by intraperitoneal injection with STZ-citric acid balance solution 50mg/kg 12 hours after fasting food but not fasting water, while the rats in the normal control group were treated by intraperitoneal injection with citric acid balance solution by the same volume. Blood samples were obtained from fossa orbitalis vein of rats 3 days after STZ was given. Hol-automatic biochemistry analyzer was used to determine FPG. The rats whose blood glucose was lower than 11.1mmol/L were rejected, and then the residual rats were randomly divided into 5 groups according to FPG and body weight: (1) Positive control group (glibenclamide 1mg/kg and mecobalamin, 0.15mg/kg); (2) High dose of Shuangqing group (4g/kg); (3) Medium dose of Shuangqing group (2g/kg); (4) Low dose of Shuangqing group (1g/kg); (5) Model group. Rats above were treated by intragastric administration with 1ml/100g at 8:30-10:00 everyday, while the ones in normal control group were done with distilled water by the same volume. The nervus coccygeus conducting velocity and thermalgia threshold should be measured 8 weeks after drugs were given.Results:1 Compared with the normal control group (43.6±0.6℃), thermalgia threshold (42.4±0.4℃) of rats in model group was raised obviously (P<0.05). Thermalgia threshold of rats in positive group was 41.9±0.9℃, which was lower than that in model group (P<0.05). In the groups treated with SQ 4g/kg and SQ 2g/kg, the thermalgia threshold was lower than that in model group (P<0.05). There was no statistical significance (P>0.05) about the thermalgia threshold of rats between SQ 1g/kg group and model group.2 Data of RM6240 electrophysiological signal collection and processing system showed: NCCV of rats in normal control group and model group was 35±6m/s and 20±3m/s, respectively. The NCCV of rats in model group was quite slow compared with that in normal group (P<0.05). There was also significant difference about NCCV of rats in positive group and that in model group (P<0.05). The NCCV of rats in SQ 4g/kg, SQ 2g/kg and SQ 1g/kg was 31±3m/s, 28±6m/s and 25±8m/s respectively. Compared with that in model group, the NCCV of rats treated with SQ 4g/kg and SQ 2g/kg was much faster (P<0.05). SQ 1g/kg did not increase NCCV significantly (P>0.05).Conclusion: SQ 2-4g/kg can increase nervus coccygeus conducting velocity and reduce the thermalgia threshold of DPN rats. |
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