Preliminary Empirical Studies On Insulin Resistance Induced By 3-deoxyglucosone And Pharmaceutical Intervention

Objective:To investigate the effect of 3-Deoxyglucosone (3-DG) on blood glucose, and on the occurrence of insulin resistance. Methods:(1) Kunming male mice were divided into two groups:3-DG treatment and control group. At day 1, week 1 and week 2 after treatment, we compared fasting blood glucose, did the glucose tolerance test and surveyed the fasting serum glucose and fasting serum insulin, calculated insulin sensitivity index (ISI). (2) Glucose oxidase-peroxidase assays were carried out to study the effect of different dose of 3-DG on the glucose consumption in HepG2 cells in vitro.(3) When observating the insulin resistant in HepG2 cell of 3-DG, eparestat,amino guanidine,puerarin,emodin,apigenin and JZ-01 were added to 3-DG in culture fluid. The influence of medicines on glucose consumption was observed.Results:(1)The results showed that administrating with 3-DG once could increase the normal mice's blood glucose immediately, administrating with 3-DG one week could result in up-moving of glucose physiological curve; administrating with 3-DG two weeks lead to the light impairment of glucose tolerance and reduction of insulin sensitivity index, and there were dose-dependence; (2)3-DG decreased HepG2 glucose consumption; (3)when HepG2 cell were incubated with 3-DG and other medicine, medicine could influence the glucose consumption which affected by 3-DG.Conclusions:The exogenous 3-DG could increase the normal mice's blood glucose. The effect of 3-DG on glucose tolerance, insulin sensitivity index and glucose consumption cue that 3-DG could induce the insulin resistance and then has relationship to the occurrence of diabetes.