Bifidobacterium Adolescent BA005 Resistance And Functional Application

The purposes of the research is to get a Bifidobacterium adolescentis from the baby's dejecta. After identification on biochemistry and molecules it would be domesticated and siftted who have strong resistance. Optimizing the condition of culture to satisfy the commercial process.Testing it's toxicity to ensure it is safety to eat. And testing it's functionality for exploitation of it.The suspected bacterial strains for purpose would be screened by the methods of diagnostic enzyme action, experiment of glucide's ferment and molecular biology testing.16S rDNA would be extracted from the bacteria and the gene order would be compared.Obtained the right bacterial and named it Bifidobacterium adolescentis BA005 by the means of Phylogenetic tree.Bifidobacterium adolescentis BA005 had been domesticated to be aerotolerant by cultivating it with and without oxygen alternately. After domestication it became strong and kept intact whether in colonial morphology or in croexamination. It growed slower than which cultured without air. The exponential phase is delayed, but bacterial count is steady. The bacterial count can be 3.30×1010 cfu/mL after 24h cultivation.The culture medium adopted maize sweet mash in order to lower costs. After optimized the condition of culture by response surface method it would be made certain that it's best condition of culture is 5% of inoculum concentration,12.1Bx of maize sweet mash's concentration, initial pH is 6.48, cultivation temperature is 37℃,fermentation time is 20h. Under this condition the OD is 1.819, viable count would be 3.05 X 1012 cfu/mL.To simulate acid and enzymes of gastrointestinal environment in order to select strong Bifidobacterium adolescentis BA005.In synthetic stomach condition survival rate of Bifidobacterium adolescentis BA005 is 72.12%; in synthetic intestinal condition survival rate is 53.33%.After testing the resistance against antibiotics, the result is that it is medium sensitivity to Amikacin, Streptomycin and Gentamicin, resistance to the others. So that it surely can work for health.Bifidobacterium adolescentis BA005 had been toxicity tested by acute toxicity test and short-term feed test with mice. Test dose was more than 10 times that people may eat, on this condition no mouse poisoned or died and no abnormalities about their internal organs. It can ensure that Bifidobacterium adolescentis BA005 is safety to eat and develop.To made diarrhea and constipation model mice by medication for comparing with different probiotics, the result is that Bifidobacterium adolescentis BA005 could cure diarrhea and constipation quicker. Bifidobacterium adolescentis BA005 has so many functions that product about it should be researched. One is fermented beverage named Kwass which is fermented by Bifidobacterium adolescentis BA005 and microzyme. It is delicious and rich nutrition. The other is functional fodder which was fermented by Bifidobacterium adolescentis BA005 and microzyme. It can promote cows assimilate nutritions, balance the microecosystem of gastrointestinal tract and improve immunity.