| Objective:Along with the rapid socio-economic development, rising living standards and acceleration of social rhythm, the phenomenon of population aging is more prominent, various types of heart disease, cerebrovascular disease now ranks among the top. According to statistics, cardiovascular disease has more than 1.8 million in China,more and more young people are constantly sick, it has become the number one killer of human health.In this sense, it is of great importance to investigate the pathomechanism of hyperlipidemia and the effective medicine to prevent and cure it.Current Western medicine for regulating blood lipids are many commonly used drugs are HMG-CoA reductase inhibitors, bile acid chelating agent, acid type, phenoxyacetic acids. Large doses of these drugs have a more positive effect, it play a role in lipid control from different aspects,but also have certain side effects such as liver damage, dissolution of striated muscle. And so far, all of the medicine, in principle, required a long-term use, thus limiting its clinical application. TCM believes that this disease is a blood disease areas of body fluid, and phlegm, blood stasis and other similar syndromes. Although the complex pathogenesis of this disease, but no more than virtual, phlegm, blood stasis, stagnation, and the spleen, liver, kidney related. We start to prevent and treat high blood lipids from the whole concept of Chinese medicine and syndrome differentiation. On the basis of previous study, we investigate the mechanism of DXR on hyperlipidemia, and study its influence of LDLR and hs-CRP, to provide guidance for clinical.When preventing and curing hyperlipidemia, TCM is characterized by multistrata, multiangular and multitarget, This study hyperlipidemia induced by high fat diet animal model, animal models for hyperlipidemia after the administration 28 days straight, test animals with hyperlipidemia and lipid parameters and test hs-CRP and LDLR levels, reveals centering side control hyperlipidemia mechanism for the development of DXR and its clinical treatment of experimental theory.Method:48 wistar rats were randomly divided into 6 groups,8 in each group, normal group, model group, DXR high dose group, DXR medium dose group, DXR low dose group and Xuezhikang Group. Normal group fed normal diet, the other group were fed high fat diet. According to body weight of rats fed, each lml/100g. The DXR group fed DXR each morning, Xuezhikang group were fed Xuezhikang, 1ml/100g (Xuezhikang 0.05g per ml), the normal group and model group were given the same volume of water, a continuous 40 days. after 40 days the rats were fasted for 12 hours, chloral hydrate anesthesia, the abdominal aorta blood, serum after centrifugation, test TG, TC, HDL, LDL levels by automatic biochemical analyzer. Enzyme-linked immunosorbent assay (ELISA) detected LDLR and hs-CRP levels.Statistically:Lots of experimental data were showed with x±s. SPSS13.0 software was applied into One-way ANOVA for mean comparison based on multiple samples. The value of P<0.05 would mean significant difference.Results:(1) Compared with normal group, the TC, TG, LDL-C levels of model group increased significantly (P=0.000), HDL-C was significantly lower (P=0.000), showed that the reproduced of high blood fat rat model was successfully.(2) In reducing the role of TC, compared with the model group, the drug group were down, but DXR low dose group was not statistically significant (P=0.322), compared with the model group, the other drug group were significantly difference (P = 0.000:0.021:0.000). DXR high dose group was significantly different compared with the medium dose group (P=0.013), DXR medium dose group was not significantly different compared with the low dose group (P=0.093).(3) In reducing the role of TG, compared with the model group, the drug group were down, but DXR low dose group was not statistically significant (P=0.285), compared with the model group, the other drug group were significantly difference (P =0.001:0.019:0.003). DXR high dose group was significantly different compared with the medium dose group (P=0.000), DXR medium dose group was significantly different compared with the low dose group (P=0.000).(4) In reducing the role of LDL-C, compared with the model group, the drug group were down, but DXR low dose group was not statistically significant (P= 0.984), compared with the model group, the other drug group were significantly difference (P=0.000:0.001:0.003). DXR high dose group was not significantly different compared with the medium dose group (P=0.773), DXR medium dose group was significantly different compared with the low dose group (P=0.001).(5) In elevated the role of HDL-C, compared with the model group, the drug group were up, the difference were significantly (P=0.000:0.000:0.001:0.000). DXR high dose group was not significantly different compared with the medium dose group (P=0.605), DXR medium dose group was significantly different compared with the low dose group (P=0.02).(6) Compared with normal group, the LDLR of the model group was significantly lower (P=0.000), compared with model group the drug group were higher, there were significant differences (P=0.000). DXR high dose group was significantly different compared with the medium dose group (P=0.001), DXR medium dose group was significantly different compared with the low dose group (P =0.000).(7) Compared with model group, the hs-CRP of the drug group was significantly lower (P=0.000). DXR high dose group was significantly different compared with the medium dose group (P=0.000), DXR medium dose group was significantly different compared with the low dose group (P=0.000).Conclusion:(1) DXR can reduce the blood lipid, the effect of the high-dose group and medium-dose group is better.(2) DXR can improve the content of LDLR of hyperlipidemic rats, therefor promote excretion and metabolism of lipid.(3) DXR can reduce the hs-CRP levels of hyperlipidemic rats, ease inflammatory reaction. |
PDF Full Text Request