A Wavelet Analysis For The Process Of Cell Rolling Adhesion

Cell rolling adhesion of thrombosis and inflammation is a very important process. Revealing this process, would help us to understand how white blood cells arrive at sites of inflammation in shear flow, why the platelets adhesion and aggregation on damaged vessel walls, as well as the relative physiological and pathological processes. It is of great significance in designing drugs for anti-inflammatory, anti-tumor, blood clots dissolving. In this thesis, the thermal response kinetics of HL-60 Cells rolling adhesion mediated by PSGL-1/E-selectin interaction was performed in flow chamber system and analyzed by wavelet analysis method. The main matter includes the three following aspects:(1) Irregularity of cells, local disturbance of flow field, experimental systematic error and other factors would cause noises in flow chamber experiment. In order to identify the real signal of cell rolling adhesion, wavelet analysis was used to segregate the noise from the original signal. It was shown that the wavelet analysis method which established by the statistical analysis of the detail signal worked very well, we can obtain the accurate time when the cell adhered and dissociated, and distinguish the processes of cell's instantaneous adhesion and instantaneous dissociation events from noise.(2) The thermal response kinetics models were built to analyze the free and tethered stage separately. The energy characteristics of thermal response kinetics in free stage was obtained by combining Langevin equation and equipartition theorem, And based on Langevin equation, an oscillator of cellular-molecular system was introduced to deduce the energy distribution for the tethered stage. In order to resolve the time scale effect driven by the emergence of the sampling time interval, a correction formula was built based on random walking, which could facilitate the analysis of data.(3) Various physical quantities were decomposed by wavelet analysis. the results figure out in the thesis that the detail of the different stages of movement was caused by a few factors. In order to find out the trends of detail in different shear stress, a dual-effect model of cell-molecular reaction system is developed, oscillator system, spacial constraint effect, thermal response kinetics model were included. Finally, according to the decomposition of the one-dimensional velocity, the energy distribution deduced from our thermal response kinetics model in tethered stage has been verified.Overall, the wavelet analysis method has been proven to be efficient in flow chamber data denoising. The thermal response kinetics models are founded to describe both the free and tethered state of cell movement, which reveals the physic process driven by molecular bond spring and thermal motion, and has been proven to be valid in this thesis.