Silane Surface Modification Of Pure Titanium With The Study Of Cytocompatibility

Titanium metal and its alloys have been widely used as dental implants, skeletal repair and orthopedic for their superior mechanical properties and good biocompatibility. However, as bio-inert material, it is difficult to form a strong chemical bonding between titanium and host bone after implantation. In addition, the oxidation film on titanium surface is too thin to prevent ion release from matrix under the physical environment and load conditions. These have seriously affected the clinical application of titanium-based implant. Therefore, it is necessary to improve its surface properties to adapt to clinical application.In this paper, the titanium plates were alkali-treatment and modified withy-aminopropyl triethoxysilane coupling agent (KH-550) to form a transition layer on it to improve the adhesion, proliferation and differentiation of cell and the healing of tissue. The biological property of the transition layer was evaluated by in-vitro test.The titanium plates were treated with alkali first to get enough Ti-OH on it. The chemical composition of the specimens were evaluated by XRD and XPS, and the morphology was characterized by SEM. It shows that there is pores structure on titanium surface after alkali-treatment, and there is a layer of TiO2 containing Ti-OH. It illustrated that the activity of the titanium was improved after alkali-treatment, and it provides the possibility to get silane surface of titanium by the condensation reaction of Ti-OH and Si-OH.They-aminopropyl triethoxysilane coupling agent (KH-550) was prepared on the titanium surface after alkali-treatment by direct impregnation and reflux condensation method. Then, the -NH2 on KH-550 could be used to bond functional groups of biological macromolecules to form bioactive and intelligent surface on metal. A in-depth research specimens properties was processed by SEM, XPS, EDX and FT-IR.The HMSCs (generation 4 to 5) were cultured on the surface of the specimens, and the biocompatibility of the specimens was evaluated by the adhesion, proliferation and differentiation of the HMSCs. It shows that the silane surface could improve the adhesion of the cells on early stage, and promote the differentiation of the HMSCs to osteoblast. The cytoskeletal protein was observed by the fluorescent immunohistochemistry method, and the result shows that the cells have a well spread on silane surface. The results of inversion fulorescence microscope, scanning electron microscopy and MTT test show that the silane titanium has good biocompatibility, and could promote the adhesion, spread, proliferation and differentiation of HMSCs. The saline titanium surface were prepared to form a transition layer in this paper, and it provide a new idea to improve the bioactivity of the titanium implantation. The transition layer made the components of the surface more reasonable at the level of molecular and cell, and maybe optimize the property of the materials surface at different stages of osseointegration.