Study On Effect Of Honey On Diabetic Wounds And Its Mechanisms

Background Diabetic foot ulcer is one of chronic complications of diabetes.It is estimated that about 15% of patients with diabetes have diabetic foot ulcer, and about 85% of patients with amputaion had diabetic foot ulcer before the amputation. Honey is an ancient wound dressing known with long history,and in recent year ,many clinical trials and animal experiments have showed that honey can be effectively treating many kinds of chronical wound inclulding diabetic foot ulcer, but it is still not answered on its details mechanism of the action.It is known that new vessel is a basical process of wound healing , bonemarrow-derived endotelia progenior cells paly a key role in this condition . It can con tribue to formaion new vessls in ulcer area that BMD-EPCs mobilize from bone marrow to peripheral blood circulation and migrate toulcer wound.SDF-1αis one key molecule which regulate this migration of the precusor cells of new vessels to damaged tissue ,and finish chemotaxis by specific binding to its receptor CXCR4.Objectives To investigate the mechanisms and therapeutic effects of honey on wound healing in Sprague-Dawley(SD) diabetic rats.Methods A round full-thickness skin wound skin lesion of 1.8cm diameter were created on the backs of streptozotocin (STZ)induced diabetes rats. Male SD rats were randomized divided into two groups :saline group(n=36) and honey group(n=36).The areas of wound and healing time are observed. Flow cytometry was assayed for CD34 and CD133 . Expression of SDF-1αwas evaluated by Real-Time PCR.Results The wound healing was quicker significantly in the rats with the honey treatment than the controlled rats on day 3 and 7[(0.38±0.12)% vs (0.26±0.12); (0.55±0.12)% vs (0.44±0.12)%] .The expression of EPCs of rats treated with honey were significantly higher on day 3 since the wound successfully made[(1.05±0.4)% vs (0.51±0.23)%].On day 3 the expression of SDF-1αwas significantly higher evaluated by Real-Time PCR.Conclusion Honey could accelerate wound healing in diabetic rats and SDF-1αcould promote endothelial cells participate in neovascularization.