The Association Of Expression Of HLA-I With The Cervical Precancerous Lesion Pathogenesis And HPV Type 16 In Uighur Women

Objective To investigate the association of loss expression of HLA-I (human leucocyte antigen class I) with the cervical precancerous lesion pathogenesis in Uighur women and high risk HPV type 16. Methods We collected 148 cases of formalin fixed and paraffin-embedded cervical tissue specimens from Uighur women with cervicitis, cervical intraepithelial neoplasia (CIN) and cervical squamous cell carcinoma. After abstracting tissue DNA from specimens,we used PCR(polymerase chain reaction, PCR) technique to detect HPV and HPV16, meanwhile we detected the protein expression level of HLA-I by SP assay of immunohistochemistry. Results The expression of HLA-I is gradually reduced in Uighur women with cervicitis, CIN I-II, CIN III,SCC. The difference among of them has statistics meaning (P<0.001). The expression of HLA-I has a negative correlation with the cervical diseases(r=-0.672, P<0.001). (2)The percentage of defect expression of HLA-I has a close relationship wih the clinical staging, the pathology differentiation level, the infiltration depth and the lymphatica metastasis. (3)the infection rate of HPV in cervicitis, CIN I-II, CIN III, cervical squamous cell carcinoma is 13%,46%,82%,95% respectively. There is a statistics difference in this four group (P<0.001), and the infection of HPV has an increasing tendency with the progression of the cervical diseases(r=0.649, P<0.001). (4) The infection rate of HPV16 in cervicitis, CIN I-II, CIN III, cervical squamous cell carcinoma is 4%,30%,68%,85% respectively. There is a statistics difference in this four group (P<0.001), and the infection of HPV16 has an increasing tendency with the progression of the cervical diseases(r=0.621, P<0.001). (5)The loss expression of HLA-I accounts 71% in the specimens in HPV16 infected. The infection of HPV16 has a negative correlation with the expression of HLA-I. Conclusion (1) The loss expression of HLA-I probably is an important signal of the cervical precancerous change, and has a great application value in the precognition and prewarning of cervical cancer. (2) The infection of HPV16 has a great promotion effect in the progress of the cervical cancer; it's a strong carcinogenesis element. (3) The relation between the infection of HPV16 and the expression of HLA-I has provided an objective evidence to reveal the morbility mechanism of cervical cancer and individualized treatment.