Resources Utilization Of The Aerial Part Of Atractylodes Macrocephala Koidz. And The Flowers Of Fritillaria Thunbergii Miq

Atractylodes macrocephala Koidz. and Fritillaria thunbergii Miq. are two traditional Chinese medicines of"Pan Wu Wei", and called"Baizhu"and"Zhebeimu", respectively. A. macrocephala. have been used as an important crude drug against stomach diseases, digestive disorders, anorexia, and the traditional custom use of this plant is the rhizome. F. thunbergii have been used as antitussive, antiasthmatic and expectorant, and the bulbs of F. thunbergii have been traditional custom used. Because of the environment of climate in Pan an, Zhejiang province is special, the quality of these two traditional Chinese medicines is much better and much more steady than any other regions'. However, most the aerial part of them are commonly disposed in landfills without any pretreatment because it was traditionally useless. Therefore, development of the aerial part as value-added products becomes a new pathway for full use of the resource of"Pan Wu Wei". In this study, we used the aerial part of A. macrocephala and the flowers of F. thunbergii as materials, and studied the chemical constituents and bioactivities of the aeral part of A. macrocephala and the flowers of F. thunbergii. The main contents of our study are as following these aspects:Part one: The chemical constituents and bioactivities of the aerial part of A. macrocephala1,Bioactivity guided isolation of anti-inflammatory fractions and was undertaken by various chromatographic methods. The active fraction-acetic ether fraction (ACE) was obtained from the 75% ethanol extract of the aerial part of A. macrocephala (EA). 25 known compounds were isolated from the active fraction, as well as 12 known compounds from n-butanol fractions, and the structures of 24 compounds were elucidated based on spectroscopic methods. Among them, 19 compounds were isolated from this species for the first time.2,A comparitive analysis of the constituents from the petroleum ether extracts of the aerial part and the rhizome of A. macrocephala was investigated by gas chromatography-mass spectrometry (GC/MS). Total of 21 compounds of the aerial part and 31 compounds of the rhizome of A. macrocephala were determined. According to our results, the main constituents of the petroleum ether extract from the aerial part were lupeol (42.18%),β-amyrin (24.45%) and oleanen-12-en-3-yl acetate (3.67%), and the extract of the rhizome obtained by petroleum ether extraction consisted mainly of 3,6-dimethyl-5-(prop-1-en-2-yl)-6-vinyl-4,5,6,7-tetrahydroben- zofuran (72.49%) and Guaia-3, 9-diene (7.12%).3,UV-Vis method for determination of total phenolic acids and total flavonoids content of the 4 parts of the aerial part of A. macrocephala. Total phenolic acids and total flavonoids content determination in different parts of A. macrocephala. showed that the acetic ether extracts is the highest.4,The investigation on antitumor activity of the petroleum ether extract from the aerial part of A. macrocephala was conducted, and it showed stronger cytotoxic activity with CEM cell line of IC50 values being below 10μg/ml. The antioxidant properties of different polarity fractions extracted from the aerial part of A. macrocephala were evaluated using DPPH radical scavenging test and superoxide anion radical scavenging activity test. It was found that the acetic ether fraction of the aerial part showed the highest antioxidant activity, compared to other fractions.Part two: The chemical constituents and bioactivities of flowers of F. thunbergii1,The acetic ether fraction (AEF) and n-butanol fractions (BEF) was obtained from the 75% ethanol extract of the flowers of F. thunbergii (EF). 13 known compounds were isolated from the active fraction, as well as 7 known compounds from n-butanol fractions, and the structures of 10 compounds were elucidated based on spectroscopic. Among them, 10 compounds were isolated from this species for the first time. Fatty oil of BMY was analyzed through GC-MS method. Thirty 25 compounds were identified, of which Linoleic acid and (9Z, 12Z, 15Z)-octadeca-9,12,15-trienoic acid(26.55 %)were the major component .2,The investigation on analgesic activity of the extract from the flowers of F. thunbergii was conducted. It was found that the acetic ether and n-butanol fractions showed the better analgesic activity, compared to other fractions.