Serum IL-15 And MIF In Severe Acute Pancreatitis And The Impact Of Traditional Chinese Medicine On Them

Objective:To investigate the roles of serum IL-15 and MIF in the early prediction of severe acute pancreatitis and to explore the mechanism of the Chaishaochengqi Decoction on severe acute pancreatitis.Methods:A total of 30 severe acute pancreatitis patients (SAP group) were randomly divided into two groups. One group (western medicine group) was treated by western medicine only. Another group (integrated tcm-wm group) was treated by combination of western medicine (wm) and traditional chinese medicine (tcm). The western medicine group treated with fasting, supplement blood-volume, keeping balance of water,electrolytes and acid-base, somatostatin, omeprazole and antibiotics. And the integrated tcm-wm group applied Chaishaochengqi Decoction besides the treatment of western medicine group. The MAP group (15 patients) was only treated with fasting, supplement blood-volume, keeping balance of water,electrolytes and acid-base. Ten healthy volunteers used as control group. Double-antibody sandwich ELISA assay was used to detect the levels of serum IL-6,IL-15,MIF on admission (1d) and 3d,5d,7d in the all AP groups and the control group. Results:1 Serum IL-6,IL-15,MIF levels in all AP patients were highest on admission.2 On admission, serum IL-6,MIF levels were highest in SAP group and lowest in control group. There were significant differences between control group and MAP group (P<0.05), and between control group and SAP group (P<0.05), and between MAP group and SAP group (P<0.05); There was no significant difference between western medicine group and integrated tcm-wm group (P>0.05).3 On admission, serum IL-15 levels were highest in SAP group and lowest in control group. There were significant differences between control group and SAP group (P<0.05), and between MAP group and SAP group (P<0.05); There were no significant differences between control group and MAP group (P>0.05), and between western medicine group and integrated tcm-wm group (P>0.05).4 On admission, serum IL-15,MIF levels were correlated positively with serum IL-6 levels and the Ranson scoring system in the SAP group.5 Duration of bellyache,abdominal distention,abdominal tenderness and the time for bowel tones to get normal in integrated tcm-wm group were shorter than western medicine group. There were significant differences between two groups (P<0.01).6 Serrum IL-6 and IL-15 levels all reduced after treatment in two SAP groups, serrum IL-6 and IL-15 levels in integrated tcm-wm group were lower than western medicine group. There were significant differences at each time point between two groups (P< 0.05).7 Serrum MIF levels all reduced after treatment in two SAP groups, serrum MIF levels in integrated tcm-wm group were lower than western medicine group. There were significant differences at 5d,7d between two groups (P< 0.05).Conclusion:1 The overexpression of serum IL-15 and serum MIF were correlated with the development of SAP, and they may have important clinical value on the early prediction of severe acute pancreatitis.2 The mechanism of Chaishaochengqi Decoction on severe acute pancreatitis may reduce the expression of serum IL-6,IL-15 and MIF.