Xiao Chai Hu Tang And Its Disassembled Prescriptions Of Anti-influenza Virus In Vitro

ObjectiveXiao Chai Hu Tang from ""Treatise on Febrile Diseases"" from Bupleurum, Scutellaria, Pinellia, ginger, ginseng, Baked Licorice, Jujube Qiwei drug composition, although all prescription Qiwei, but combined with appropriate, prescriptions strict, with cold and heat, and use, reinforcement and facilities, coordinated movements, reconciliation, Shao Yang, Shu Li triple burner, tune up air machines, announced through internal and external, the role of Cardinal running. Commonly used in modern clinical respiratory disease and infectious diseases, such as:upper respiratory tract infection, influenza, unexplained fever, mumps, epidemic conjunctivitis. Influenza is caused by the influenza virus, acute respiratory infection, can cause a worldwide pandemic. Recent years the flu virus from spreading, the urgent need for new anti-influenza drugs, and influenza virus antigen has been variation, making it difficult to play a good role in Western medicine. The innovation of this issue is that traditional Chinese medicine theory and combining modern science and technology, in the early in vivo based on the use of serum pharmacology and cell culture technology, and further in vitro experiments, and the compatibility of Xiao Chai Hu Tang Group inhibition of influenza virus research Xiaochaihu decoction and its mechanism of anti-influenza virus, as well as compatibility between the mechanism of pharmacological synergy. This study was to reveal the compatibility of the treatment by the side of science and rigor, promote Chinese traditional Chinese medicine, to explore the motherland treasure trove of traditional Chinese medicine for clinical use provided by a strong theoretical basis, are of great significance.MethodsThe experimental application of serum pharmacology and cell culture techniques to study the compatibility of Xiao Chai Hu Tang and the inhibition of cell toxicity and the role of influenza virus. Xiao Chai Hu Tang observed side group and its full compatibility group of lung adapted strain of influenza virus in vitro. Specific methods are as follows:1,16 the Wistar rats were randomly divided into eight groups, each group fed with the corresponding drug preparation containing serum to prepare for the latter part of experiment.2, pig kidney cells in conventional preservation, mass generation, determination of influenza virus titer obtained virus TCID50, to prepare for later experimental use.3, drug-containing serum on cell toxicity test:The eight group-containing serum were added to cell suspension in 3 days after the MTT method, 570nm wavelength colorimetric measuring OD values of serum containing different concentrations on cell growth in order to experiment to determine the next step by adding the amount of drug-containing serum. 4, in vitro antiviral activity observed: The 100TCID of influenza A virus solution was inoculated into the swine kidney cells grow into a monolayer on the set 37℃,5%CO2 incubator, within 2 hours after absorption. Carefully absorb the virus solution, washed three times with PBS were added to final concentration of 10% of the 8 group-containing serum, each set of four parallel holes. Drugs and Cells 3 days, MTT colorimetric method 570nm wavelength measured OD values to calculate percentage inhibition.Results1, comparison of each group containing different concentrations of serum on swine kidney cells Toxicity can be seen, each group of drugs with the drug-containing serum showed an increase in cell proliferation and growth. However, all groups except in 10% of the normal serum concentration and no differences, at 5%,20%,40% of the concentration of serum with normal control group were different. May be due to the low proportion of serum, each group containing serum drug concentration in blood too low; a high proportion of serum, the blood concentration has already reached saturation, and serum globulin in complement or components under the action of other substances can produce cell drug trafficking in the cell's prejudice. In order to facilitate comparison the following experiments to determine experimental drug-containing serum concentration of 10%.2, Xiao Chai Hu Tang Fang-wide group of influenza virus significantly inhibited, while other parties split the group contains Bupleurum, Scutellaria each group was significantly higher than inhibition of influenza virus does not contain Bupleurum, Scutellaria each group, indicating that XIAOCHAIHU soup can effectively inhibit influenza virus, Bupleurum, Scutellaria baicalensis in anti-influenza virus played an important role inConclusionUsing serum pharmacological research on Chinese anti-viral compound in vitro experiments, in vitro experimental conditions, strong controllability. Easy detection of drug effects can be further reveal the mechanism of drug action; consolidated results of this study, Xiao Chai Hu Tang has obvious anti-influenza virus effects, drug side effects, its role is better than the split party; in Xiao Chai Hu Tang decoction in order to Chai Qin compatibility of the various groups of anti-influenza virus compound the role of the strongest, indicating that in the recipe, the main component of Bupleurum, Scutellaria part in anti-influenza virus infection may play a major role, ginger, Pinellia, ginseng, jujube, Zhigancao of influenza virus is not significantly inhibited, but not entirely in the overall antiviral activity has no effect, its mechanism remains to be explored in the next step experiments.