The Effect Of Polysaccharides From The Seed Of Plantago Asiatica L. And Tea Glycoprotein On The Fuctions Of Murine Macrophage Cells

Macrophage is an important immune effector cells with immune defense and with regulating many immune functions, by phagocytosis of microorganisms and secreting a variety of cytokines. Other functions include regulating the body's inflammatory immune response in the immune system. Macrophages can express more than a few dozens of receptors, resulting in more than ten kinds of enzymes. It has a strong phagocytic function, through taking the initiative deformation movement, and eating particles of extra antigen. The secretion of NO and tumor necrosis factor (TNF-α) and other molecules directly kill target cells, while it producing a variety of biological active substances in order to play a strong role in immune regulation.In our nature, plant species are various. Amoung these natural plants, the plants with immune regulation still are huge. In order to develop the function food in the future, we would study the physical and chemical properties of its active ingredients.In this study, macrophages as the vitro model for studying of tea polysaccharide complex (Tea glycoprotein, TGP), and plantain seed polysaccharide complex (Polysaccharide isolated from the seed of Plantago asiatica L., PL-PS).The results are as follows:1. Observation of cell morphology, we observe the characterization of the morphology. Cell shape and size showed that the two polysaccharides on macrophage have no toxic effects.We can conclusion thar experimental samples can be used in next experiments.2. Through observing the effects of different doses of PL-PS and TGP on RAW264.7 and murine peritoneal macrophages function, Griess and ELISA in this experiment were detected on RAW264.7 and murine peritoneal macrophages. We found that PL-PS and TGP can induce RAW264.7 and murine peritoneal macrophages produce NO and cytokines TNF-α, IL-1β. Thus, PL-PS and TGP can promote RAW264.7 and murine peritoneal macrophages NO and cytokines productiong through regulating different types of immune responses.3. In this study, we used flow cytometry technique and neutral red test to analyze the phagocytosis and the surface of CD80, CD86 and CD40 expression levels. The results showed that PL-PS and TGP can promote CD80, CD86 and CD40 expression. The function in control group is weak, but stimulated by different doses of PL-PS, TGP-the phagocytic activity were enhanced.PL-PS, TGP can activated macrophages may be related to enhanced phagocytosis and surface molecular expression of the two kinds of cells.4. Using RT-PCR method, we study TNF-α, IL-1βmRNA expression. It showed that the group of total RNA extracted, the group of P-actin expression in RAW264.7, can be used as internal reference gene; TNF-α, IL-1βmRNA expression was increased by stimulated by TGP.These results suggest that PL-PS and the TGP can promote macrophage CD80, CD86 and CD40 expression, and enhance their phagocytosis, NO and cytokine of TNF-α, IL-1βsecretion. And through the promotion TNF-α, IL-1βmRNA of macrophage expression, macrophages is activated.