Establishment Of Mice Myelomonocytic Leukemia Model And The Study Of Immunoprotection For Allogeneic Hematopoietic Cells By FK506 And CsA

[Objective]The mice model of myelomonocytic leukemia is established. Based on the model, the mechanism of immunoprotection and the influence on allogeneic hematopoietic cells'survival of FK506 and CsA are researched.[Method]Two WEHI-3 cells injection methods, caudal vein injection and Qiuhou injection, are used respectively to establish the myelomonocytic leukemia (MML) model. After WEHI-3 cells are injected into mice, some symptomatic comparisons are made between the two injection methods, which are survival conditions, peripheral blood cells counting and bone marrow examinations. The purpose of comparisons is to select a better injection method. The hematopoietic cells gotten from the green fluorescent protein (GFP) transgenetic ICR mice are cultured and used as the receptor cells to infuse the MML mice. FK506 and CsA are injected respectively into the model mice with GFP hematopoietic cells, and then the influence of FK506 and CsA treatment on the GFP hematopoietic cells'survival as well as the IL-2/IL-4 expression level of receptor mice are researched. [Results]Compared with the mice by Qiuhou injection, those by caudal vein injection have a lower mortality, a longer life cycle and a more evident symptom. On the fourteenth day after injection, the percentage of immature granulocyte and immature myelomonocytic starts to rise. On the twentieth day, the injected mice meet the myelomonocytic leukemia criterion. By the twenty-eighth day, the percentage of bone marrow immature myelocyte have risen obviously, which means that the models are established successfully and caudal vein injection is better than Qiuhou injection. GFP hematopoietic cells in the host with FK506 or CsA treatment can not only survive at least two weeks but also have better survival conditions, while they can survive only one week without treatment. In addition, both FK506 and CsA treatment can reduce the levels of IL-2 and IL-4 in leukemia mice, and FK506 has a better effect.[Conclusion]Caudal vein injection is a better method than Qiuhou injection in establishing the myelomonocytic leukemia model. Both CsA and FK506 can improve the survival of GFP hematopoietic cells in host mice by reducing the levels of IL-2 and IL-4, and FK506 is preferable. The mechanism may be that repressing of the expression levels of IL-2 and IL-4 can immunoprotect allogene hematopoietic cell and increase their survival rate in transplanting.