Association Between Toll-like Receptor 2,4 Polymorphisms And Leprosy

Objective:To study the relationship between Toll-like receptor2(TLR2) (597T/C),TLR4(896A/G) single nucleotide polymorphisms(SNP) and leprosy, and the genetic susceptibility of leprosy. Methods:A total 117 cases(from Bijie district, Guizhou Province, China)diagnosed as leprosy[divided into lepromatous type(LL,79 cases) and tuberculoid type(TT,38 cases) or familial aggregations(43 cases) and non-familial aggregations(74 cases)] and 50 healthy controls were enrolled; polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) was applied to analyze the genotypes of TLR2(597T/C),4(896A/G) of all samples, percentages of different genotypes between all patients and controls,LL and TT,familial aggregations and non-familial aggregations were compared. Theχ2 test was applied in statistical analysis. Results:The frequency of TLR2(597T/C) genotype TT,TC,CC was 46.2%,40.2%,13.6% and 48.0%,42.0%,10.0% in all patients and controls respectively, no significance was found(χ2= 0.431,P>0.05); The frequency of genotype TT,TC,CC was 44.3%,40.5%,15.2% and 50.0%,39.5%,10.5% in LL and TT respectively,48.8%,37.2%,14.0% and 44.6%,41.9%,13.5% in familial aggregations and non-familial aggregations respectively, both are no significance(χ2=0.595,0.258;P>0.05). We did not find TLR4(896A/G) polymorphism in all subjects including patients and controls,the genotype was all 896AA homozygous wild. Conclusion:TLR2(597T/C),TLR4(896A/G) polymorphisms are not related to leprosy from Bijie, Guizhou,China, TLR4(896T/C) polymorphism is rare in China.