| ObjectiveTo explore the effect and the mechanism of HBO on renal IRI and to provide the theory for prevention and cure of renal IRI through HBO in clinical application by observing apoptosis of the rats with renal IRI and the effect of HBO on apoptosis through the renal IRI model in rats.Material and Methods60 adult healthy Wistar rats were divided into 4 groups randomly:normal group (n=18), sham operation group (n=18), control group of renal IRI (n=18), HBO treated group of renal IRI (n=18). The rats in posterior three groups were divided into 3 subgroups according the time:1 h after reperfusion,3 h after reperfusion,5 h after reperfusion. Each subset contained 6 rats. The right kidney of the rats in all groups was excised and the left kidney was observed. The left kidney of the rats in normal group was excised immediately when the right kidney was excised. The left renal artery was blocked in the control group of renal IRI and the HBO treated group of renal IRI while the left renal artery was not blocked in the sham operation group. In HBO treated group of renal IRI, the rats were put in hyperbaric chamber for 1 h at the time of ischemia/reperfusion,2 h after reperfusion,4 h after reperfusion where the pressure was 0.1 MPa and the oxygen concentration was 98% and above. The content of the BUN, Scr, MDA and TNF-a in blood serum in all groups was detected at 1h,3h,5h after reperfusion. And the left kidney was used in paraffin section and HE stain, FCM, electron microscope and detection of apoptosis which was double staining of Amnnexin V and PI. Results1. In control group of renal IRI, the levels of BUN, Scr, MDA and TNF-a in blood serum were significantly higher than those in normal group and sham operation group (P<0.05), and those in control group of renal IRI were significantly higher than those in HBO treated group of renal IRI at the different phase(P<0.05).2. FCM detection revealed that there were few apoptosis in renal tissues in normal group and sham operation group while lots of apoptosis in control group of renal IRI. There were significant differences in the expression of apoptosis between control group of renal IRI and HBO treated group of renal IRI (P<0.05).3. There was a positive correlation between the levels of BUN, Scr, MDA, TNF-a in serum and apoptosis in renal tissues.4. It was shown that in 4 group, predominant renal tubular epithelial cell vacuole degeneration disruption,and necrosis, and the injury aggravated with ischemia-reperfusion time increasing. the glomerular capillary more alleviated congestive than group 3, renal tubular epithelial cell showed approximately normal structure, alleviated vacuole degeneration after HBO treatment.Conclusions1 The manufacture of the oxygen free radical and proinflammatory factor in blood may enhance the apoptosis after the renal IRI and so that the injury of the kidney was aggravated.2 The important reason of the renal functional lesion after IRI is that the manufacture of the oxygen free radical and proinflammatory factor induces the apoptosis of cells in the nephridial tissue.3 The important mechanism of the protection of HBO in renal IRI is that HBO restrain the manufacture of the oxygen free radical and proinflammatory factor, and also the restrain of the apoptosis.4 The early application of HBO is more favourable for relieving renal IRI. |
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