Expression And Clinical Significance Of HTERC And HER-2 Gene In Cervical Scraping Samples

Objective To explore the expression and their clinical significance of hTERC and HER-2 gene and to evaluate the effect and clinical significance of HER-2 and hTERC in cervical lesion.Methods The fluorescent in situ hybridization (FISH) was used to detect the expression of the hTERC and HER-2 gene in cervical scraping samples and the Paraffin-embedded tissue sections with five Production Methods. The cervical scraping samples were divided into 45 in normal,29 in CIN I,38 in CINâ…¡-â…¢and 50 in cervical cancer. The Paraffin-embedded tissue sections were divided into 13 in normal,12 in CIN I,20 in CIN II-III and 18 in cervical cancer.Results We detected 225 specimens, which including 12 cases of direct smear method,60 cases of saline method,20 cases of low osmotic method,70 cases of TCT production method and 63 cases of paraffin-embedded tissue sections method. The total hybridization success rate is 76.4%; The achievement ratio of the hybridization of hTERC and HER-2 gene for the direct smear method, the saline method, the TCT production method, the low osmotic method and the Paraffin-embedded tissue section method were 8.3%,65%,55%,87.1% and 85.7 %,respectively. The achievement ratio of TCT production method was higher than that of the other four group. The low osmotic method is better than others in the background of the cleanliness, the cell morphology and the number of the bare nuclei. The Paraffin-embedded tissue section method is the best in the satisfaction rate of the fluorescence signal. The hTERC and HER-2 gene in 40 cases of normal cervix was not expressed, while in other lesions were expressed. The hTERC gene in CIN I group, CINâ…¡-â…¢group, cervical cancer in the group positive expression rate are 16.1%,47.6% and 91.5% and the difference was statistically significant (P<0.05); The HER-2 gene in CIN I group, CIN II-III group, cervical cancer in the group positive expression rate are 6.5%,28.6% and 59.3% and the difference was statistically significant (P<0.05). The hTERC and HER-2 gene overexpression significantly associated with cell grade (P<0.05). And the hTERC and HER-2 gene in cervical carcinoma with tumor lymph node metastasis and differentiation of the degree of correlation (P<0.05). But the patient's age, tumor size, clinical stage, histological type and pathological types were non-significant correlation (P> 0.05). Conclusion The TCT production method is better than other methods in the hTERC and HER-2 gene detecting of the cervical cancer screening. Otherwise, during the treatment guidance of the cervical lesions and the cervical cancer the Paraffin-embedded tissue section method is least at the damage of the chromosome and is the best at the practical significance. The hTERC and HER-2 gene were highly expressed in the occurrence and development of cervical cancer They may play an important role in cervical lesions detected in cervical carcinogenesis.The hTERC and HER-2 gene of cervical scraping samples could be used as a better tumor index for early cervical cancer screening and predicate the advancement and evaluate poor prognosis of cervical lesion.