Effects Of Irradiation Of Intense Pulsed Light On The Collagen Synthes In Vitro And In Vivo

ObjectivesTo investigate the effects of intense pulsed light(IPL)on the collagen metabolism in mouse skin and human fibroblasts; partially elucidate molecular mechanisms of IPL action.Methods1.The depilated mice were exposed to the dose of 32J/cm2 Intense Pulsed Light (IPL), At the time of 1,7,14and 28d, the skin specimen were collected, the collagen synthesis were determined by hematoxylin-eosin-staining and Van Gieson (V.G) staining.2.The human fibroblasts cultured in vitro were exposed to the dose of 10,18,27,36and36 J/cm2x2IPL. The type of I and III collagen synthes of fibroblasts was measured determinedby immunohistochemical Method.Results1.exposure to 32J/cm2 doses of IPL irradiation only leads a slight hyperdermis (P>0.05),but induces overexpression of collagen in mouse skin(P<0.05).2.contrasted with the sham-irradiation,IPL irradiation showed no noticeable effect on the type ofâ… andâ…¢collagen synthes of human fibroblasts(P>0.05).ConclusionIPL irradiation can enhance the content of collagens in mouse skin, but can not significantly increase the activity of collagen synthes ecreting of human fibroblasts.