Different Concentrations Of Hepatocyte Growth Factor And Fibroblast Growth Factor In The Regulation Of Hepatic Stem Cells In Rats

Different concentrations of hepatocyte growth factor and fibroblast growth factor in the regulation of hepatic stem cells in ratsObjectiveTo evaluate effect of different concentrations of hepatocyte growth factor (HGF) and fibroblast growth factor (FGF) on the regulation of hepatic stem cell proliferation. Methods Hepatic stem cells from rats were cultured under different conditions (Group A and B). HGF was used in Group A, with the concentrations of 1,5,10,20,40,80, and 100 ng/mL. Cytokines was not used in the control group FGF was used in Group B, with the concentrations of 1,5,10,20,40,80, and 100 ng/mL The best concentration of HGF and FGF was to obtain via the analysis of the dose-response relationship in Groups A and B. Then hepatic stem cells were cultured in Group C, with the optimal concentration of HGF joint 1,5,10,20,40,80, and 100 ng/mL FGF. Mononuclear cell direct cytotoxicity assay (MTT) was used to analyze the proliferation effect of hepatic stem cells intervened by HGF and FGF. Results The result shows that different concentration of HGF and FGF could promote cell prolferration, and the effect of prolferration is dose-dependent. The best concentration of HGF and FGF was 20 ng/mL and 10 ng/mL. Conclusion: BothHGH and FGF can accelerate the proliferation of hepatic stem cells in vitro, which has a synergistic action..