Study On Flavonoids In Flowers Of Salix Caprea .l In Xinjiang And Its Extraction Technique Of Flavonoids

Salix caprea of Xinjaing is widely distributed id the Xinjiang Uyghur Autonomous region of China . This medicinal herb has physiological activities such as anti-free radical , antioxidant, antivirus, anti- tumor, invigoration of eyes, abirritation. Response Surface Method (RSM) was applied to optimize the extracting condition for total flavonoids from Salix caprea by supersonic wave. The optimum process for supersonic extraction were 63.88 % ethanol, 21.45min supersonic time, 1∶35.94 mass-liquid ratio. And we had studied on its chemical compounds of Salix caprea.L.Salix caprea flowers was extracted with alcohol(70%), and distributed by petroleum ether, ether acetate, n-buthanol ,respectively.Isolated and purified via silica gel column chromatography, Sphadex LH-20,HPLC.Then ,12 compounds were isolated and 9 compounds were identified as :β-sitosterol,(+)-catechin, quercetin, kaempferol-3-О-β- D- glucopyranoside,apigenin-7-О-β-D-glucopyranoside,3'-methoxyl-kaempferol-7-О-β-D- glucopyranoside,luteolin-7-О-β-D- glucopyranoside,Isorhamnetin -3-O-α-L-rhamnosyl-(1→6)-β-D-glucoside and Rutin.