The Study Of Formaldehyde's Genotoxicity On Spider Cells And Comparison Of Formaldehyde's Genotoxicity On Different Animal Cells

Indoor formaldehyde (FA) is considered one of the most important air pollutants in the world, which raises concerns in the field of environment and human health. To thoroughly explore the toxicity of FA, especially its genotoxicity that can lead even canner, is with significant meaning of improving the indoor air quality which can also be used as scientific evidence when the government evaluates air pollution and establishes safety levels of environment FA.1. FA Genotoxicity on spider cellsThe genotoxicity of FA on ecological environment was studied in this research. Eriovixia cavaleriei, one kind of spider that only can be found in China, was exposed in gaseous FA (0,0.5,1.0,3.Omg-m-3). Single cell gel electrophoresis and KCl-SDS assay were applied to detect the DNA damage, including DNA strand breaks (DSB) and DNA-protein crosslink (DPC). DNA damage was found in all three tested concentrations of gaseous FA with dose-effect responses. DSB was observed with low and medium concentrations of FA exposure, while DDC and DPC with high concentration.2. Comparison of FA Genotoxicity on different animal cellsOn the basis of the measurement that calculates gaseous FA concentration in 0.9% NaCl solution, concentrations of gaseous FA were converted to liquid FA. Its genotoxicity on different animal cells were compared and analyzed using biomarkers DSB, DDC and DPC. With increasing exposure concentration, DSB, DDC and DPC were observed in sequence, and the otherness of sensitivity of different animal cells on FA exposure also increased; FA genotoxicity varied in different kinds of animal cells, or even in different organs from the same animal; FA genotoxicity present a more complex quality in vertebrate cells.