| Objective:Gastrointestinal (GI) fistulas are frequently very serious complications that are associated with high morbidity and mortality. GI fistulas can cause a wide array of pathophysiological effects by allowing abnormal diversion of the GI contents, including digestive fluid, water, electrolytes, and nutrients, from either one intestine to another or from the intestine to the skin. Conservative management of GI fistulas is a valuable therapy that includes drainage and several other procedures. A combination of various methods for controlling intestinal output and decreasing the amount of infection will increase the chances of a successful outcome._Heme oxygenase-1 (heme oxygenase-1, HO-1), also known as heat shock protein 32 (hot shock protein 32, HSP32), is rate-limiting enzyme in the process of heme degradation, can be induced by a variety of factors such as inflammatory factors, oxidation, ischemia, hypoxia, endotoxin, etc.. Heme oxygenase-1 (HO-1) as a highly conserved stress protein, can protect the organs, tissues and cells to resist injury caused by a variety of stimulating factors and pathological process. rAAV vectors have become increasingly recognized as a safe and efficient vehicle for gene therapy with multiple advantages over other therapeutic gene delivery systems, owing to their wide range of infection, low levels of inducing immune responsiveness, and potential for long term gene expression. Mesenchymal (stromal) stem cells (MSC) are rare population of precursor cells capable of maintaining hemopoiesis and differentiating in vivo and in vitro into three (osteoblasts, chondrocytes, adipocytes) or more(myocytes, cardiomyocytes, neurons, etc.) cell types. That is why MSC attract much attention as candidates for replacement or reparative therapy of diseases, gene or cell engineering. Several laboratories have studied MSCs in tissue repair models. The results from these experiments have been mixed:often, MSCs have been shown to express differentiated markers in very low numbers accompanied by a measurable therapeutic effect, or a therapeutic effect is present but no differentiation is detected. In the case of severe tissue ischemia or damage, MSCs can be attracted to the damaged site, where they secrete bioactive factors that trophically influence the repair and regenerative process. MSCs can be mobilized from the marrow or can be expanded in culture and delivered to the damaged site by direct or systemic injection. Once at the site of injury, MSCs produce factors that inhibit scarring and apoptosis, promote angiogenesis and stimulate host progenitors to divide and differentiate in order to repair the injured tissue. In this regard, the trophic effects of MSCs may have important clinical use. The aim of this study was to investigate the effect of MSC encoding HO-1 on colocuteneous fistulas in rats.Methods:The isolation and culturing of rat MSC. MSC was transfected by Recombined adeno-associated Viral Vector 2 encoding HO-1. Two groups were assigned:Group A, Injecting 1×106 MSC encoding HO-1 in colocuteneous fistulas in rats 24 hours postoperation; Group B, as control group. The experimental data were expressed with (x±s) and analysed by SPSS 12.0 statistic software.Results:The healing of Group A is faster than contralConclusions:MSC encoding HO-1 is effective in the healing of colocuteneous fistulas in rats. |
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